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Ultrastructural localization of actin in normal human skin

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Summary

Normal human skin was embedded in Lowicryl K4M. Actin microfilaments were localized by applyinga postembedding immunogold technique using the monoclonal anti-actin antibody HHF35. Actin microfilaments are part of the cytoskeleton in muscle and nonmuscle cells. Together with myosin they produce contraction. The antibody labelled myofilaments in smooth muscle arrector pili cells, myoepithelial cells and pericytes. In sweat gland cells the microvilli system, a zone beneath the cytoplasma membrane correponding to the adhesion belt region, and apocrine decapitation formations showed labelling.

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References

  1. Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson J (1989) Molecular biology of the cell. Garland Publishing, New York/London

    Google Scholar 

  2. Carlemalm R, Garavito M, Villinger W (1982) Resin development for electron microscopy and an analysis of embedding in low temperature. J Microsc 126: 123–143

    Google Scholar 

  3. Drenckhalm D, Dermietzel R (1988) Organization of the actin filament cytoskeleton in the intestinal brush border: a quantitative and qualitative immunoelectron microscope study. J Cell Biol 107: 1037–1048

    Google Scholar 

  4. Hirokava N, Hemser JE (1981) Quick freeze, deep-etch visualization of the cytoskeleton beneath surface differentiations of intestinal epithelial cells. J Cell Biol 91: 399–409

    Google Scholar 

  5. Lazarides E, Weber K (1974) actin antibody: the specific visualization of actin filaments in non-muscle cells. Proc Natl Acad Sci USA 71: 2268–2272

    Google Scholar 

  6. Lever WF, Schaumburg-Lever G (1990) Histopathology of the skin, 6th edn. Lippincott, Philadelphia London Mexico City

    Google Scholar 

  7. Tsukada T, Tippens D, Gordon D, Ross R, Gown AM (1987) HHF35, a muscle-actin-specific monoclonal antibody I. Immunocytochemical and biochemical characterization. Am J Pathol 126: 51–60

    Google Scholar 

  8. Tsukada T, McNutt MA, Ross R, Gown AM (1987) HHF35, a muscle actin-specific monoclonal antibody II. Reactivity in normal, reactive and neoplastic human tissues. Am J Pathol 127: 389–402

    Google Scholar 

  9. Vandekerckhove J, Weber K (1978) At least six different actins are expressed in a higher mammae. J Mol Biol 126: 783–802

    Google Scholar 

  10. Yoshihara T, Kanda T, Nagata H, Nomoto M, Kaneko T, Kato Y, Yaku Y (1988) Cytochemical demonstration of actin filaments in myoepithelial cells of the human parotid gland. Acta Anat. 132: 317–320

    Google Scholar 

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Metzler, G., Schaumburg-Lever, G., Fehrenbacher, B. et al. Ultrastructural localization of actin in normal human skin. Arch Dermatol Res 284, 242–245 (1992). https://doi.org/10.1007/BF00375802

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  • DOI: https://doi.org/10.1007/BF00375802

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