Summary
We have studied the embryonic and adult phenotypes of genetic combinations between Polycomb (Pc), Regulator of bithorax (Rg-bx) and the genes of the Bithorax complex (BX-C) and the Antennapedia complex (ANT-C). The products of Pc and Rg-bx genes act antagonistically, their mutant combinations leading to the ectopic expression of genes of the BX-C and ANT-C. The genetic analysis of the Pc locus suggests it is a complex gene. Pc+ products behave as members of a “regulatory set” that negatively control the expression of BX-C and ANT-C genes. Genetic combinations between different doses of Pc, Rg-bx and the genes of the BX-C and ANT-C have phenotypes which may be interpreted as resulting from ectopic derepression of posterior selector genes repressing selector genes of anterior segments. The transformation phenotypes of certain genetic combinations differ in embryos and adults. A model of regulation of the BX-C and the ANT-C genes during the imaginal cell proliferation is presented, in which the specification state is maintained by self-activation of a given selector gene and down modulation of other selector genes in the same cell.
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References
Akam ME (1983) The location of Ultrabithorax transcripts in Drosophila tissue sections. EMBO J 2:2075–2084
Beachy PA, Helfland SL, Hogness DS (1985) Segmental distribution of bithorax complex proteins during Drosophila development. Nature 313:545–551
Bender W, Weiffenbach B, Karch F, Peifer M (1985) Cold Spring Harbor Symp Quant Biol 50:173:180
Botas J, Moscoso del Prado J, García-Bellido A (1982) Gene-dose titration analysis in the search of transregulatory genes in Drosophila. EMBO J 1:307–310
Cabrera CV, Botas J, García-Bellido A (1985) The specificity of distribution of Ultrabithorax proteins in mutants of the Bithorax Syntagma. Nature 318:569–571
Capdevila MP, Garciá-Bellido A (1978) Phenocopies of Bithorax mutants. Wilhelm Roux's Arch 185:105–126
Capdevila MP, García-Bellido A (1981) Genes involved in the activation of the Bithorax Complex of Drosophila. Wilhelm Roux's Arch 190:339–350
Denell RE (1973) Homoeosis in Drosophila: I. Complementation studies with revertants of Nasobemia. Genetics 75:279–297
Duncan I (1982) Polycomb-like: a gene that appears to be required for the normal expression of the Bithorax and Antennapedia gene complexes of Drosophila melanogaster. Genetics 102:49–70
Duncan I, Kaufman TC (1975) Cytogenetic analysis of chromosome 3 in Drosophila melanogaster: mapping of the proximal portion of the right arm. Genetics 80:733–752
Duncan I, Lewis EB (1982) Genetic control of body segment differentiation in Drosophila. In: Subtelny S (ed) Developmental order: its origin and regulation. Alan R. Liss, New York, pp 533–554
Dura JM, Brock HW, Santamaría P (1985) Polyhomeotic: a gene of Drosophila melanogaster required for correct expression of segmental identity. Mol Gen Gent 198:213–220
Gans M, Forquignon F, Masson M (1980) The role of dosage of the region 7D1–7D5.6 of the X chromosome in the production of homoeotic transformations in Drosophila melanogaster. Genetics 96:887–902
García-Bellido A (1975) Genetic control of wing disc development in Drosophila. In: Brenner S (ed) Cell patterning. Ciba Symposium 29. Elsevier, Amsterdam, pp 161–182
García-Bellido A (1982) The bithorax syntagma. In: Lakovaara S (ed) Advances in genetics, development and evolution. VII Europ. Dros. Research Conf. Plenum Press, New York, pp 135–148
García-Bellido A (1983) Comparative anatomy of cuticular patterns in the genus Drosophila. In: Goodwin BC, Holder N, Wylie CG (eds) Development and evolution. Cambridge Univ. Press, Cambridge, pp 227–255
García-Bellido A, Capdevila MP (1978) The initiation and maintenance of gene activity in a developmental pathway of Drosophila: In: Subtelny S, Sussex IM (eds) Clonal basis of development. Academic Press, New York, pp 3–21
Hafen E, Levine M, Gehring WJ (1984) Regulation of Antennapedia transcript distribution by the bithorax complex in Drosophila. Nature 307:287–289
Hall CJ, Kankel DR (1976) Genetics of acetyl cholinesterase in Drosophila melanogaster. Genetics 83:517–535
Ingham PW (1981) Trithorax: a new homoeotic mutation of Drosophila melanogaster. II. The role of trx+ after embryogenesis. Wilhelm Roux's Arch 190:365–369
Ingham PW (1983) Differential expression of bithorax complex genes in the absence of the extra sex comb and trithorax genes. Nature 306:591–593
Ingham PW (1984) A gene that regulates the bithorax complex differentially in larval and adult cells of Drosophila. Cell 37:815–823
Ingham PW (1985) A clonal analysis of the requirement for the trithorax gene in the diversification of segments in Drosophila. J Embryol Exp Morphol 89:349–365
Ingham PW, Whittle R (1980) Trithorax: a new homoeotic mutation of Drosophila melanogaster causing transformations of abdominal and thoracic imaginai segments. Mol Gen Genet 179:607–614
Jürgens G (1985) A group of genes controlling the spatial expression of the bithorax complex in Drosophila. Nature 316:153–155
Kaufman TC (1978) Cytogenetic analysis of chromosome 3 in Drosophila melanogaster: isolation and characterization of four new alleles of the proboscipedia (pb) locus. Genetics 90:579–596
Kaufman TC, Lewis R, Wakimoto B (1980) Cytogenetic analysis of chromosome 3 in Drosophila melanogaster. The homoeotic gene complex in polytene chromosome interval 84A-B. Genetics 94:115–133
Lawrence PA, Johnston D, Struhl G (1983) Different requirements for homoeotic genes in the soma and germ line of Drosophila. Cell 35:27–34
Lewis EB (1968) Genetic control of developmental pathways in Drosophila melanogaster. Proc. 12th. Int. Congr. Genet 1:96–97
Lewis EB (1978) A gene complex controlling segmentation in Drosophila. Nature 276:565–570
Lewis EB (1980) Report on new mutants of Drosophila melanogaster. DIS 55:207–208
Lindsley DL, Grell EH (1968) Genetic variations of Drosophila melanogaster. Carnegie Institut Washington Publ. N° 627
Lindsley DL, Sandler E, et al. (1972) Segmental aneuploidy and the genetic gross structure of the Drosophila genome. Genetics 71:157–184
Miñana FJ, García-Bellido A (1982) Preblastoderm mosaics of mutants of the bithorax complex. Wilhelm Roux's Arch 191:331–334
Morata G (1975) Analysis of gene expression during the development on the homoeotic mutant Contrabithorax of Drosophila. J Embryol Exp Morphol 34:19–31
Morata G, Kerridge S (1981) Sequential functions of the bithorax complex of Drosophila. Nature 290:778–781
Nüsslein-Volhard C, Wieschaus E (1980) Mutations affecting segment number and polarity in Drosophila. Nature 287:795–801
Puro J, Nygrén T (1975) Mode of action of a homoeotic gen in Drosophila melanogaster. Hereditas 81:237–248
Sánchez-Herrero E, Morata G (1983) Genetic and developmental characteristics of the homoeotic mutation bx1 of Drosophila. J Embryol Exp Morphol 76:251–264
Sánchez-Herrero E, Vernós I, Marco R, Morata G (1985) Genetic organization of Drosophila bithorax complex. Nature 313:108–113
Sato T, Russell MA, Denell RE (1983) Homoeosis in Drosophila: a new enhancer of Polycomb and related homoeotic mutations. Genetics 105:357–370
Struhl G (1981a) A gene product required for correct initiation of segmental determination in Drosophila. Nature 293:36–41
Struhl G (1981b) A homoeotic mutations transforming leg to antenna in Drosophila. Nature 292:635–638
Struhl G (1982) Genes controlling segmental specification in the Drosophila thorax. PNAS 79:7380–7384
Struhl G (1983) Role of the esc + gene product in ensuring the selective expression of segment-specific homoeotic genes in Drosophila. J Embryol Exp Morphol 76:297–331
Struhl G, Akam M (1985) Altered distributions of Ultrabithorax transcripts in extra sex comb mutant embryos of Drosophila. EMBO 14:3259–3264
Struhl G, White RA (1985) Regulation of the Ultrabithorax gene of Drosophila by other bithorax complex genes. Cell 43:507–519
Van der Meer JM (1977) Optical clean and permanent whole mount preparations for phase contrast microscopy of cuticular structures of insect larvae. DIS 52:160
Wakimoto BT, Kaufman TC (1981) Analysis of larval segmentation in lethal genotypes associated with the Antennapedia gene complex in Drosophila melanogaster. Dev Biol 81:51–64
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Capdevila, M.P., Botas, J. & García-Bellido, A. Genetic interactions between the Polycomb locus and the Antennapedia and Bithorax complexes of Drosophila . Roux's Arch Dev Biol 195, 417–432 (1986). https://doi.org/10.1007/BF00375746
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DOI: https://doi.org/10.1007/BF00375746