Abstract
Urodilatin, a renal natriuretic peptide that is an analogue to circulating atrial natriuretic peptide [α-ANP (99-126)], is measurable with a highly specific and sensitive radioimmunoassay. While most ANP antibodies cannot distinguish between urodilatin and other ANP analogues, the polyclonal urodilatin antibody specifically measures human urodilatin without any cross-reactivity to other ANP analogues. Urodilatin is not detected in blood from healthy volunteers nor from cardiac patients. Urinary urodilatin accounts for only a part of total urinary ANP immunoreactivity. Urodilatin excretion closely parallels sodium excretion in response to an acute volume load while changes in urinary immunoreactive ANP excretion do not reflect this renal response. We conclude that specific urodilatin assays are required to explore further the physiological role of the renal natriuretic peptide.
Similar content being viewed by others
References
Bub A, Rose M, Mägert HJ, Forssmann WG (1992) Urodilatin-like immunoreactivity (URO-IR) and urodilatin gene expression in mammalian kidney (abstract). Histochem J (Suppl) 24:517
Drummer C, Fiedler F, König A, Gerzer R (1991) Urodilatin, a kidney-derived natriuretic factor, is excreted with a circadian rhythm and is stimulated by saline infusion in man. J Am Soc Nephrol 1:1109–1113
Drummer C, Gerzer R, Heer M, Molz B, Bie P, Schlossberger M, Stadaeger C, Röcker L, Strollo F, Heyduck B, Bauer K, Warberg J, Baisch F, Christensen N-J, König A, Norsk P (1992) Effects of an acute saline infusion on fluid and electrolyte metabolism in humans. Am J Physiol 262:F744-F754
Emmeluth C, Drummer C, Gerzer R, Bie P (1992) Roles of cephalic sodium concentration and urodilatin in control of renal sodium excretion. Am J Physiol 262:F513-F516
Forssmann WG, Nokihara K, Gagelmann M, Hock D, Feller S, Schultz-Knappe P, Herbst F (1989) The heart is the center of a new endocrine, paracrine, and neuroendocrine system. Arch Histol Cytol 52 [Suppl]: 293–315
Goetz KL (1991) Renal natriuretic peptide (urodilatin?) and atriopeptin: evolving concepts. Am J Physiol 261:F921-F932
Goetz KL, Drummer C, Zhu JL, Ledley R, Fiedler F, Gerzer R (1990) Evidence that urodilatin, rather than ANP, regulates renal sodium excretion. J Am Soc Nephrol 1:867–874
Heim J-M, Kiefersauer S, Fülle H-J, Gerzer R (1989) Urodilatin and beta-ANF: binding properties and activation of particulate guanylate cyclase. Biochem Biophys Res Commun 163:37–41
Kentsch M, Ludwig D, Drummer C, Gerzer R, Müller-Esch G (1992) Haemodynamic and renal effects of urodilatin in healthy volunteers. Eur J Clin Invest 22:319–325
de Léan A, Vinay P, Cantin M (1985) Distribution of atrial natriuretic factor receptors in dog kidney fractions. FEBS Lett 193:239–242
Morris BJ (1976) Specific radioactivity of radioimmunoassay tracer determined by self-displacement: a re-evaluation. Clin Chim Acta 73:213–216
Ritter D, Needleman P, Greenwald JE (1991) Synthesis and secretion of an atriopeptin-like protein in rat kidney cell culture. J Clin Invest 87:208–212
Saxenhofer H, Raselli A, Weidmann P, Forssmann WG, Bub A, Ferrari P, Shaw SG (1990) Urodilatin, a natriuretic factor from kidneys, can modify renal and cardiovascular function in men. Am J Physiol 259:F832-F838
Schulz-Knappe P, Forssmann K, Herbst F, Hock D, Pipkorn R, Forssmann WG (1988) Isolation and structural analysis of “urodilatin”, a new peptide of the cardiodilatin-(ANP)-family, extracted from human urine. Klin Wochenschr 66:752–759
Schulz-Knappe P, Honrath U, Forssmann WG, Sonnenberg H (1990) Endogenous natriuretic peptides: effect on collecting duct function in rat kidney. Am J Physiol 259:F415-F418
Tremblay J, Gerzer R, Vinay P, Pang SC, Béliveau R, Hamet P (1985) The increase in cGMP by atrial natriuretic factor correlates with the distribution of particulate guanylate cyclase. FEBS Lett 181:17–22
Valentin J-P, Sechi LA, Qiu C, Schambelan M, Humphreys MH (1991) Urodilatin displaces labeled atrial natriuretic peptide from renal binding sites and is equally potent in generating cyclic GMP (abstract). Hypertension Dallas 18:393
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Drummer, C., Fiedler, F., Bub, A. et al. Development and application of a urodilatin (CDD/ANP-95#x2013;126)-specific radioimmunoassay. Pflügers Arch. 423, 372–377 (1993). https://doi.org/10.1007/BF00374930
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00374930