Abstract
Effects of 2,3-butanedione-2-monoxime (BDM) on the contraction of intact and skinned smooth muscles from guinea-pig portal vein were examined. In intact preparations loaded with fura-2, 5–10 mM BDM markedly suppressed Ca2+ transients and force developments induced by 154 mM potassium and by phenylephrine (0.1 mM). On the other hand, in Ca2+-free depolarizing solution, BDM did not suppress phenylephrine (0.1 mM)-induced Ca2+ transient and force development. In skinned preparations obtained with Staphylococcus aureus α-toxin treatment, BDM did not markedly affect active force development. The above results indicate that BDM suppresses contraction of the portal vein mainly by the inhibition of voltage-dependent cytosolic Ca2+ transients. An additional result suggests that BDM suppresses the force-enhancing effect of α 1-adrenergic agents on the contractile elements.
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Watanabe, M. Effects of 2,3-butanedione monoxime on smooth-muscle contraction of guinea-pig portal vein. Pflugers Arch. 425, 462–468 (1993). https://doi.org/10.1007/BF00374873
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DOI: https://doi.org/10.1007/BF00374873