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Effects of endurance training on myosin heavy-chain isoforms and enzyme activity in the rat diaphragm

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Abstract

We investigated the effects of endurance training (20 m/min, 60 min/day, 5 days/week) on myosin heavy-chain (MHC) isoforms and succinic dehydrogenase (SDH) activity in rat crural and costal diaphragms, and plantaris muscles. Although the 4-week endurance training produced significant (P<0.05) increases, both in SDH activity and the percentage of isoform HCIIa in the plantaris of the trained rat compared with the sedentary control rat, these alterations did not occur in either the crural or costal diaphragms. After 10 weeks of endurance training, trained animals had significantly (P<0.05) higher SDH activity in the costal diaphragm and the plantaris. Moreover, a significant (P<0.05) decrease occurred in the percentage of HCIIb in the costal diaphragm, and a significant (P<0.01) decrease in the percentage of HCIIb concomitant with a significant (P<0.05) increase of HCIIa resulted in the plantaris. However, the crural diaphragm did not show any significant changes after 10 weeks of endurance training. These results indicate that endurance training induces an alteration in the expression of an MHC phenotype, in addition to causing an increase in oxidative enzyme activity. However, the alterations in response to endurance training are apparently not uniform, varying between regions and/or kinds of muscles.

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Authors and Affiliations

  1. Laboratory of Biomechanics and Physiology, Faculty of Liberal Arts, Yamaguchi University, 753, Yamaguchi, Japan

    Takao Sugiura

  2. Department of Physiology, Yamaguchi University School of Medicine, Ube, 755, Yamaguchi, Japan

    Akio Morimoto & Naotoshi Murakami

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  1. Takao Sugiura
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  2. Akio Morimoto
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  3. Naotoshi Murakami
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Sugiura, T., Morimoto, A. & Murakami, N. Effects of endurance training on myosin heavy-chain isoforms and enzyme activity in the rat diaphragm. Pflügers Arch 421, 77–81 (1992). https://doi.org/10.1007/BF00374736

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  • DOI: https://doi.org/10.1007/BF00374736

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