Abstract
Ethanol was produced with Zymomonas mobilis Z6 (ATCC 29191), in batch culture with synthetic medium on glucose as substrate and in the presence of aspartate. The concentrations of glucose, phosphate, ammonium, ethanol and dissolved O2 and CO2 in the medium and O2 and CO2 in the outlet gas as well as the cell mass by culture fluorescence were measured on-line. Cell mass, glucose and aspartate concentrations were measured off-line. In the presence of a sufficient amount of aspartate, the ethanol inhibition effect can be reduced considerably. However, the improvement with yeast extract is more incisive. The relationship between the intensity of culture fluorescence and cell mass concentration is linear, if sufficient aspartate is present.
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Abbreviations
- ASP kg/m3 :
-
aspartate concentration
- CTR kg/(m3 · h):
-
CO2 transfer rate
- N, NH4 kg/m3 :
-
nitrogen concentration from NH +4
- P kg/m3 :
-
product (ethanol) concentration
- p%:
-
product (ethanol) yield
- PO4 kg/m3 :
-
phosphate concentration
- Q E kg/(kg · h):
-
specific ethanol production rate
- \(Q_{{\text{N,NH}}_{\text{4}} } \) kg/(kg · h):
-
specific nitrogen uptake rate from NH +4
- Q P kg/(kg · h):
-
specific phosphate uptake rate
- Q s kg/(kg · h):
-
specific substrate (glucose) uptake rate
- S kg/m3 :
-
glucose concentration
- S O kg/m3 :
-
initial glucose concentration
- Y x/s kg/kg:
-
yield coefficient
- μ h−1 :
-
specific growth rate
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Schmidt, W., Scheper, T. & Schügerl, K. Ethanol production with Zymomonas mobilis with synthetic medium in batch operation. Bioprocess Engineering 3, 129–133 (1988). https://doi.org/10.1007/BF00373476
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DOI: https://doi.org/10.1007/BF00373476