Abstract
In the present study, covalently bound lipids were found in clavus material and their lipid classes were determined by high-performance thin-layer chromatography (HPTLC). Clavus material, pulverized completely in a Mikro-Dismembrator II, was exhaustively extracted three times with chloroform/methanol (2∶1, 1∶1 and 1∶2 v/v) at 80‡C for l h each time and this sequence of extractions was repeated to obtain the unbound lipid-free residue which was saponified and then extracted with chloroform. The extract proved to comprise several bands of lipids covalently bound through an ester-like linkage. These were identified as free fatty acids, cholesterol, ceramides and glucocerebrosides by HPTLC. However, Ω-hydroxy fatty acids were not detected in the lipids. To analyse the fatty acids amide-linked to the bound ceramides, the latter were isolated by preparative HPTLC and subjected to mild acid hydrolysis. Since the bound ceramides constituted neither Ω-hydroxy fatty acids nor α-hydroxy fatty acids, they were not identified as hydroxyl-acylsphingosines.
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Serizawa, S., Ito, M., Hamanaka, S. et al. Bound lipids liberated by alkaline hydrolysis after exhaustive extraction of pulverized clavus. Arch Dermatol Res 284, 472–475 (1993). https://doi.org/10.1007/BF00373359
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DOI: https://doi.org/10.1007/BF00373359