Abstract
The class II major histocompatibility complex antigens are cell-surface heterodimers consisting of an a and a β chain. Cosmid cloning has shown that the three families of clas II antigens, DR, DQ, and DP, are encoded within the HLA-D region of chromosome 6 as a series of discrete gene clusters. The DP cluster contains two pairs of a and β genes, one of which encodes the biochemically-defined DP antigen. In order to assess whether the other two genes, SXa and SXβ, are also expressed, potential coding regions have been subcloned and sequenced. The SX3β gene is shown to contain region closely homologous to all six exons of DPβ. A 1 bp deletion in the β2 exon, also observed for the SX4β allele, causes a translation frameshift, suggesting that SXβ is a pseudogene. However, all the other exons, as well as their splice sites and the putative promoter region, appear to be intact.
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Benacerraf, B.: Role of MHC gene products in immune regulation. Science 212: 1229–1238, 1981
Bodmer, W. F., Albert, E., Bodmer, J. G., Dausset, J., Kissmeyer-Nielsen, F., Mayr, W., Payne, R., van Rood, J. J., Trnka, Z., and Walford, R. L.: Nomenclature for factors of the HLA system 1984. Immunogenetics 20: 593–601, 1984
Boss, J. M., Mengler, R. A., Okada, K., Auffray, C., and Strominger, J. L.: Sequence analysis of the human major histocompatibility gene SXα. Mol. Cell. Biol. 5: 2677–2683, 1985
Dodgen. J. B. and Engel, J. D.: The nucleotide sequence of the adult chicken α globin gene. J. Biol. Chem. 258: 4623–4628, 1983
Gustafsson, K., Emmoth, E., Widmark, E., Bohme, J., Peterson, P. A., and Rask, L.: Isolation of a cDNA clone coding for an SB β chain. Nature 309: 76–78, 1984
Hurley, C., Shaw, S., Nadler, L., Schlossman, S., and Capra, J.: Alpha and beta chains of SB and DR antigens are structurally distinct. J. Exp. Med. 156: 1557–1562, 1982
Inoko, H., Ando, A., Kimura, H., Ogata, S., and Tsuji, K.: Isolation and characterization of the cDNA clones and the genomic clones of the HLA class II antigen heavy chains. Ninth Histocompatibility Workshop. In E. D. Albert, M. P. Baur, and W. R. Mayr (eds.): Histocompatibility Testing 1984, pp. 559–564, Springer-Verlag, Berlin, 1984
Kappes, D. J., Arnot, D., Okada, K., and Strominger, J. L.: Structure and polymorphism of the HLA class II SB light chain genes. EMBO J. 3: 2985–2993, 1984
Kaufman, J. F., Auffray, C., Korman, A. J., Shackelford, D. A., and Strominger, J. L.: The class II molecules of the human and murine major histocompatibility complex. Cell 36: 1–13, 1984
Korman, A. J., Boss, J. M., Spies, T., Sorrentino, R., Okada, K., and Strominger, J. L.: Genetic complexity and expression of human class II histocompatibility antigens. Immunol. Rev. 85: 45–86, 1985
Krangel, M. S.: Unusual RNA splicing generates a secreted form of HLA-A2 in a mutagenized B lymphoblastoid cell line. EMBO J. 4: 1205–1210, 1985
Larhammar, D., Hyldig-Nielsen, J., Servenius, B., Andersson, G., Rask, L., and Peterson, P. A.: Exon-intron organization and complete nucleotide sequence of a major histocompatibility antigen DCβ gene. Proc. Natl. Acad. Sci. U.S.A. 80: 7313–7317, 1983
Larhammar, D., Servenius, B., Rask, L., and Peterson, P. A.: Characterization of an HLA-DRβ pseudogene. Proc. Natl. Acad. Sci. U.S.A. 82: 1475–1479, 1985
Long, E., Wake, C., Gorski, J., and Mach, B.: Complete sequence of an HLA-DRβ chain deduced from a cDNA clone and identification of multiple nonallelic DRβ chains. EMBO J. 2: 389–394, 1983
Mathis, D., Benoist, C., Williams, V., Kanter, M., and McDevitt, H. D.: The murine Eα immune response gene. Cell 32: 745–754, 1983
Maxam, A. M. and Gilbert, W.: Sequencing end-labelled DNA with base-specific chemical cleavages. Methods Enzymol. 65: 499–560, 1980
Mount, S. M.: A catalogue of splice junction sequences. Nucleic Acids Res. 10: 459–472, 1982
Okada, K., Boss, J. M., Prentice, H., Spies, T., Mengler, R. A., Auffray, C., Lillie, J., Grossberger, D., and Strominger, J. L.: Gene organization of the DC and DX subregions of the human major histocompatibility complex. Proc. Natl. Acad. Sci. U.S.A. 82: 3410–3414, 1985a
Okada, K., Prentice, H. L., Boss, J. M., Levy, D. J., Kappes, D. J., Spies, T., Raghupathy, R., Mengler, R. A., Auffray, C., and Strominger, J. L.: SB subregion of the human major histocompatibility complex: Gene organization, allelic polymorphism and expression in transformed cells. EMBO J. 4: 739–748, 1985b
Pawelec, G., Shaw, S., and Wernet, P.: Analysis of the HLA-linked SB gene system with cloned and uncloned alloreactive T-cell lines. Immunogenetics 15: 187–198, 1982
Saito, H., Maki, R., Clayton, L., and Tonegawa, S.: Complete primary structures of the Eβ chain and its gene of the mouse major histocompatibility complex. Proc. Natl. Acad. Sci. U.S.A. 80: 5520–5524, 1983
Shaw, S., Kavathas, P., Pollack, S., Charmot, D., and Mawas, C.: Family studies define a new histocompatibility locus, SB, between HLA-DR and GLO. Nature 293: 745–747, 1981
Spies, T., Sorrentino, R., Boss, J. M., Okada, K., and Strominger, J. L.: Structural organization of the DR subregion of the human major histocompatibility complex. Proc. Natl. Acad. Sci. U.S.A. 82: 5165–5169, 1985
Torczynski, R. M., Fuke, M., and Bollon, A. P.: Human genomic library screened with 17-base oligonucleotide probes yields a novel interferon gene. Proc. Natl. Acad. Sci. U.S.A. 81: 6451–6455, 1984
Trowsdale, J., Kelly, A., Lee, J., Carson, S., Austin, P., and Travers, P.: Linkage map of two HLA-SBβ and two HLA-SBα-related genes: An intron in one of the SBβ genes contains a processed pseudogene. Cell 38: 241–249, 1984
Vieira, J. and Messing, J.: The pUC plasmids, an M 13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19: 259–268, 1982
Widera, G. and Flavell, R. A.: The I-region of the C57BL/10 mouse: Linkage to H-2K and characterization of a novel, SBβ-like, class II pseudogene, Aβ3. Proc. Natl, Acad. Sci. U.S.A. 82: 5500–5504, 1985
Wieringa, B., Meyer, F., Reiser, J., and Weissman, C.: Unusual splice sites revealed by mutagenic inactivation of an authentic splice site of the rabbit β-globin gene. Nature 301: 38–43, 1983
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Kappes, D.J., Strominger, J.L. Structure and evolution of the HLA Class II SXβ gene. Immunogenetics 24, 1–7 (1986). https://doi.org/10.1007/BF00372291
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DOI: https://doi.org/10.1007/BF00372291