Abstract
UVA- and UVB-induced alterations in dermal collagen were investigated in a murine animal model. Groups of hairless mice were exposed to UVA and UVB for 28 weeks at a dose of 60 J/cm2 three times weekly and 0.06 J/cm2 three times weekly, respectively. Untreated animals were used as controls. Every 4 weeks dorsal skin was examined for quantitative and qualitative changes in dermal collagen. Neither UVA nor UVB caused a significant alteration in total skin collagen content. However, after UVA treatment the ability of skin collagen to be digested by pepsin decreased dramatically (up to 65% of skin collagen remained insoluble after 4 months), whereas exposure to UVB had no significant effect. Furthermore a shift in the ratio of α1(I,III) chains to α2(I) chains was detected after UVA exposure. The amount of type V collagen in mouse skin, as determined by a sensitive ELISA method, was markedly decreased after UVA treatment, but not after UVB treatment.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bailey AJ, Robins SP, Balian G (1974) Biological significance of the intermolecular crosslinks of collagen. Nature 39: 347–350
Barksy SH, Rao CN, Grotendorst GR, Liotta LA (1982) Increased content of type V collagen in desmoplasia of human breast cancer. Am J Pathol 108: 276–283
Boyer B, Fourtanier A, Kern P, Labat-Robert J (1992) UVA- and UVB-induced changes in collagen and fibronectin biosynthesis in the skin of hairless mice. J Photochem Photobiol B 14: 247–259
Johnston KJ, Oikarinen AI, Lowe NJ, Clark JG, Uitto J (1984) Ultraviolet radiation-induced connective tissue changes in the skin of hairless mice. J Invest Dermatol 82: 587–590
Kligman LH, Gebre M (1989) Biochemical changes in collagen after chronic UVA radiation. Photochem Photobiol 49s : 18s (abstract)
Kligman LH, Gebre M (1991) Biochemical changes in hairless mouse skin collagen after chronic exposure to ultraviolet-A radiation. Photochem Photobiol 54: 233–237
Kligman LH, Akin FJ, Kligman AM (1985) The contributions of UVA and UVB to connective tissue damage in hairless mice. J Invest Dermatol 84: 272–276
Kligman LH, Gebre M, Alper R, Kefalides NA (1989) Collagen metabolism in ultraviolet irradiated hairless mouse skin and its correlation to histochemical observations. J Invest Dermatol 93: 210–214
Laemmli UK (1970) Cleavage and structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685
Marian B, Danner MW (1987) Skin tumor promotion is associated with increased type V collagen content in the dermis. Carcinogenesis 8: 151–154
Marian B, Mazzucco K (1985) Dermal collagen metabolism during tumor promotion with 12-O-tetradecanoylphorbol-13-acetate in mouse skin. Carcinogenesis 6: 501–504
Mutzhas MF, Hoelzle E, Hofmann C, Plewig G (1981) A new apparatus with high radiation energy between 320–460 nm: physical description and dermatological applications. J Invest Dermatol 76: 42–47
Plastow SR, Harrison JA, Young A (1988) Early changes in dermal collagen of mice exposed to chronic UVB irradiation and the effects of a UVB sunscreen. J Invest Dermatol 91: 590–592
Ramshaw JAM, Werkmeister JA (1988) Electrophoresis and electroblotting of native collagens. Anal Biochem 168: 82–87
Rennard SI, Berg R, Martin GR, Foidart JM, Gehron Robey P (1980) Enzyme-linked immunoassay (ELISA) for connective tissue components. Anal Biochem 104: 205–214
Schwartz E (1988) Connective tissue alterations in the skin of ultraviolet irradiated hairless mice. J Invest Dermatol 91: 158–161
Schwartz E, Cruikshank FA, Perlish JS, Fleischmajer R (1989) Alterations in dermal collagen in ultraviolet irradiated hairless mice. J Invest Dermatol 93: 142–146
Trautinger F, Trenz A, Raff M, Kokoschka EM (1989) Influence of UV radiation on dermal collagen content in hairless mice. Arch Dermatol Res 281: 144 (abstract)
Woessner JF (1961) The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid. Arch Biochem Biophys 93: 440–447
Woodley DT, Scheidt VJ, Reese MJ, Paller AS, Manning TO, Yoshiike T, Briggaman RA (1987) Localization of the alpha 3 (V) chain of type V collagen in the skin. J Invest Dermatol 88: 246–252
Yamauchi M, Prisayanah P, Haque Z, Woodley DT (1991) Collagen cross-linking in sunexposed and unexposed sites of aged human skin. J Invest Dermatol 97: 938–941
Zheng P, Kligman LH (1993) UVA-induced ultrastructural changes in hairless mouse skin: a comparison to UVB-induced damage. J Invest Dermatol 100: 194–199
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Trautinger, F., Mazzucco, K., Knobler, R.M. et al. UVA- and UVB-induced changes in hairless mouse skin collagen. Arch Dermatol Res 286, 490–494 (1994). https://doi.org/10.1007/BF00371578
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00371578