Abstract
The myosin heavy chain (MHC) composition of single fibres from m. vastus lateralis was analysed by one-dimensional electrophoresis and immunoblotting in three groups of young men with distinct difference in physical activity patterns. No major co-existence of MHC isoforms was found in the group with some daily physical activity. In the very sedentary group, however, 19±5% (P<0.05) of the fibres exhibited coexistence of MHC type IIa and IIb. Further, in the endurance trained group co-existence of MHC type I and IIa was manifested in 36±4% (P<0.05) of the fibres. Disuse and extreme usage of muscle both give rise to an elevation in co-expression of MHC isoforms in single muscle fibres but of markedly different combination of isoforms.
This is a preview of subscription content, log in via an institution to check access.
References
Baumann H, Jaggi M, Soland F, Howald H, Schaub MC (1987) Exercise training induces transitions of myosin isoform subunits within histochemically typed human muscle fibres. Pflügers Arch 409: 349–360
Biral D, Betto R, Danieli-Betto D, Salviati G (1988) Myosin heavy chain composition of single fibres from normal human muscle. Biochem J 250: 307–308
Carraro U, Catani C (1983) A sensitive SDS-PAGE method separating myosin heavy chain isoforms of rat skeletal muscles reveals the heterogeneous nature of the embryonic myosin. Biochem Biophys Res Comm 116: 793–802
Danieli-Betto D, Zerlato E & Betto R (1986) Type 1, 2A and 2B myosin heavy chain electrophoretic analysis of rat muscle fibers. Biochem Biophys Res Comm 138(2): 981–987
Green HJ, Klug GA, Reichmann H, Seedorf U, Wiehrer W, Pette D (1983) Exercise-induced fibre type transition with regard to myosin, parvalbumin, and sarcoplasmic reticulum in muscles of the rat. Pflügers Arch 400: 432–438
Klitgaard H, Zhou M, Schiaffino S, Betto R, Salviati G & Saltin B (1990) Ageing alters the myosin heavy chain composition of single fibres from human skeletal muscle. Acta Physiol Scand, in press
Pette D, Vrbova G (1985) Invited review: Neural control of phenotypic expression in mammalian muscle fibres. Muscle & Nerve 8: 676–689
Schantz PG (1986) Plasticity of human skeletal muscle. Acta Physiol Scand Vol 128, Supplementum 558
Staron RS, Gohlsh B, Pette D (1987) Myosin polymorphism in single fibres of chronically stimulated rabbit fast-twitch muscle. Pflügers Arch 408: 444–450
Staron RS, Pette D (1986) Correlation between myofibrillar ATPase activity and myosin heavy chain composition in rabbit muscle fibers. Histochemistry 86: 19–23
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Klitgaard, H., Bergman, O., Betto, R. et al. Co-existence of myosin heavy chain I and IIa isoforms in human skeletal muscle fibres with endurance training. Pflügers Arch 416, 470–472 (1990). https://doi.org/10.1007/BF00370757
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00370757