Abstract
Single-channel currents were recorded from two classes of Ca2+ channels in visceral smooth muscle cells isolated from the stomach of the toad, Bufo marinus: a class of small-conductance channels (approximately 11 pS) and a class of large-conductance channels (approximately 26 pS). Small-conductance channels were present in a majority of patches and gave rise to a slowly inactivating current (t1/2 ≈ 250 ms at 0 mV). Openings of large-conductance channels could be unequivocally resolved only in the presence of the dihydropyridine Ca2+ agonist Bay K 8644. Two subtypes of the large-conductance channels were found — those with a very slow rate of decay (> 500 ms) and those with a faster one (< 100ms). Large-conductance channels resemble L-type Ca2+ channels of other preparations. Small-conductance channels do not fit unambiguously into the other existing categories (i.e., N or T). Correspondence between single-channel and macroscopic Ca2+ currents is discussed.
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Aosaki T, Kasai H (1989) Characterization of two kinds of voltage-activated Ca-channel currents in chick sensory neurons. Pflügers Arch 414:150–156
Bean BP (1989) Classes of calcium channels in vertebrate cells. Ann Rev Physiol 51:367–384
Bean BP, Sturek M, Puga A, Hermsmeyer K (1986) Calcium channels in muscle cells isolated from rat mesenteric arteries: modulation by dihydropyridine drugs. Circ Res 59:229–235
Benham CD, Hess P, Tsien RW (1987) Two types of calcium channels in single smooth muscle cells from rabbit ear artery studied with whole-cell and single-channel recordings. Circ Res 61:I10-I16
Bossu JL, Fagni L, Feltz A (1989) Voltage-activated calcium channels in rat Purkinje cells maintained in culture. Pflügers Arch 414:92–94
Cavalié A, Pelzer D, Trautwein W (1986) Fast and slow gating behaviour of single calcium channels in cardiac cells: relation to activation and inactivation of calcium-channel current. Pflügers Arch 406:241–258
Clapp LH, Vivaudou MB, Walsh JV Jr, Singer JJ (1987) Acetylcholine increases voltage-activated Ca2+ current in freshly dissociated smooth muscle cells. Proc Natl Acd Sci USA 84:2092–2096
Clapp LH, Vivaudou MB, Singer JJ, Walsh JV Jr (1989) Substance P, like acetylcholine, augments one type of Ca2+ current in isolated smooth muscle cells. Pflügers Arch 413:565–567
Eckert R, Chad JE (1984) Inactivation of Ca channels. Prog Biophy Mol Bol 44:215–267
Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflügers Arch 391:85–100
Hess P, Lansman JB, Tsien RW (1985) Different modes of Ca channel gating behaviour favoured by dihydropyridine Ca agonists and antagonists. Nature 311:538–544
Inoue Y, Xiong Z, Kitamura K, Kuriyama H (1989) Modulation produced by nifedipine of the unitary Ba current of dispersed smooth muscle cells of the rabbit ileum. Pflügers Arch 414:534–542
Kass RS (1987) Voltage-dependent modulation of cardiac calcium channel current by optical isomers of Bay K 8644: implications for channel gating. Circ Res 61 [Suppl I]:I1-I5
Kitamura K, Inoue Y, Inoue R, Ohya Y, Terada K, Okabe K, Kuriyama H (1989) Properties of the inward ionic currents and their regulating agents in smooth muscle cells. Gen Physiol Biophys 8:289–312
Lassignal NL, Singer JJ, Walsh JV Jr (1986) Multiple neuropeptides exert a direct effect on the same isolated smooth muscle cell. Am J Physiol 250:C792-C798
Nowycky MC, Fox AP, Tsien RW (1985) Three types of neuronal calcium channel with different calcium agonist sensitivity. Nature 316:440–443
Tsien RW, Lipscombe D, Madison DV, Bley KR, Fox AP (1988) Multiple types of neuronal calcium channels and their selective modulation. Trends Neurosci 11:431–438
Vivaudou MB, Clapp LH, Walsh JV Jr, Singer JJ (1987) Multiple types of Ca2+ current in single smooth muscle cells and their regulation by substance P and acetylcholine. J Gen Physiol 90:42a
Vivaudou MB, Clapp LH, Walsh JV Jr, Singer JJ (1988) Regulation of one type of Ca2+ current in smooth muscle cells by di-acylglycerol and acetylcholine. FASEB J 2:2497–2504
Vivaudou MB, Singer JJ, Walsh JV Jr (1989) Single-channel and whole-cell recordings of two types of Ca2+ currents in gastric smooth muscle cells: effects of the dihydropyridine Bay K 8644. J Gen Physiol 94:14a-15a
Worley JF III, Deitmer JW, Nelson MT (1986) Single nisoldipine-sensitive calcium channels in smooth muscle cells isolated from rabbit mesenteric artery. Proc Natl Acad Sci USA 83:5746–5750
Yamamoto Y, Hu SL, Kao CY (1988) Demonstration of multiple types of calcium channels in smooth muscle cells of the guinea-pig taenia coli. Biophys J 53:594a
Yatani A, Seidel CL, Allen J, Brown AM (1987) Whole-cell and single-channel calcium currents of isolated smooth muscle cells from saphenous vein. Circ Res 4:523–533
Yoshino M, Nishio A, Yabu H (1988a) Calcium channel modulation by the dihydropyridine derivative Bay K 8644 in mammalian visceral smooth muscle cells. Comp Biochem Physiol 89C:39–43
Yoshikno M, Someya T, Nishio A, Yabu H (1988b) Whole-cell and unitary Ca channel currents in mammalian intestinal smooth muscle cells: evidence for the existence of two types of Ca channels. Pflügers Arch 411:229–231
Yoshino M, Someya T, Nishio A, Kazawa K, Usuki T, Yabu H (1989) Multiple types of voltage-dependent Ca channels in mammalian intestinal smooth muscle cells. Pflügers Arch 414:401–409
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Vivaudou, M.B., Singer, J.J. & Walsh, J.V. Multiple types of Ca2+ channels in visceral smooth muscle cells. Pflügers Archiv 418, 144–152 (1991). https://doi.org/10.1007/BF00370463
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DOI: https://doi.org/10.1007/BF00370463