Abstract
Enzyme production with E. coli ATCC 11105, in a complex medium using phenylacetic acid as inducer is carried out in a stirred-tank reactor of 10 dm3 and an airlift tower-loop reactor of 60 dm3 with outer loop at a temperature of 27 °C. The optimum inducer concentration was 0.8 kg/m3, which was kept constant by fed-batch operation. The optimum of the relative dissolved O2-concentration with regard to saturation is below 10% in a stirred-tank reactor and at 35% in a tower-loop reactor. It was kept constant by parameter-adaptive control of the aeration rate. In a stirred-tank enzyme productivity is slightly higher than in a tower-loop reactor, and much higher than in a bubble column reactor.
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Abbreviations
- CPR kg/(m3 h):
-
CO2-production rate
- OTR kg/(m3 h):
-
O2-transfer rate
- OUR kg/(m3 h):
-
O2-utilization rate
- PAA:
-
phenylacetic acid (inducer)
- RQ = CPR/OUR:
-
respiratory quotient
- X kg/m3 :
-
cell mass concentration
- μ m h−1 :
-
maximum specific growth rate
References
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Gebauer, A., Scheper, T. & Schügerl, K. Penicillin acylase production by E. coli. Bioprocess Engineering 2, 55–58 (1987). https://doi.org/10.1007/BF00369523
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DOI: https://doi.org/10.1007/BF00369523