Abstract
Cross-flow filtration (CFF) has been investigated as a method of separating filamentously growing fungal cells and purifying the polysaccharide produced. The effects of transmembrane pressure, module geometry (e.g. channel height or tube diameter), tangential feed velocity and cell as well as polysaccharide concentration are discussed. Apart from these experiments, influences by the recirculation pump used are shown.
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Abbreviations
- b f :
-
fouling index
- b :
-
factor refering to the behaviour of the sublayer
- C kg · m−3 :
-
concentration
- C g kg · m−3 :
-
solute concentration at the membrane
- C b kg · m−3 :
-
solute concentration in the bulk phase
- D s-1 :
-
shear rate
- k m · s−1 :
-
mass-transfer coefficient
- K mPa · sn :
-
consistency index
- n :
-
flow behaviour index
- P w m3 · s−1 · m−2 :
-
rate of permeation
- P w1 m3 · s−1 · m−2 :
-
rate of permeation at 1 minute
- P′ w m3 · s−1 · m−2 :
-
rate of permeation at the beginning
- p :
-
Pa pressure
- Q m2 :
-
largest cross-section of a particle
- q m2 :
-
smallest cross-section of a particle
- Re :
-
Reynolds number
- R f −1 :
-
fouling resistance
- R m m−1 :
-
membrane resistance
- t s:
-
time
- w m · s−1 :
-
tangential feed velocity
- λ :
-
friction factor
- ΔpTM Pa:
-
transmembrane pressure
- η mPa · s:
-
shear viscosity
- η sp :
-
specific viscosity (rel. increase of viscosity ηsp=ηrel-1)
- [η] m3· kg−1 :
-
intrinsic viscosity
- ν w m2 · s−1 :
-
kinematic viscosity
- ϱ kg · m−3 :
-
density
- b :
-
bulk
- cell :
-
cells
- f :
-
fouling
- g :
-
gelling
- PS :
-
polysaccharide
- rel :
-
relative
- sp :
-
specific
- w :
-
water
References
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Haarstrick, A., Rau, U. & Wagner, F. Cross-flow filtration as a method of separating fungal cells and purifying the polysaccharide produced. Bioprocess Eng. 6, 179–186 (1991). https://doi.org/10.1007/BF00369256
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DOI: https://doi.org/10.1007/BF00369256