Abstract
A nylon wool separation technique was employed to separate rainbow trout leucocytes into adherent and non-adherent populations. The non-adherent population showed a greater response to concanavalin A (ConA) and a lesser response to lipopolysaccharide (LPS) than did the adherent population in the spleen, kidney and peripheral blood. The great majority (>90%) of thymocytes were in the non-adherent population. The non-adherent population from the spleen, kidney and peripheral blood showed signicantly (P>0.05) higher numbers of acid phosphatase-positive lymphocytes than the adherent population, but there was no significant difference in the pattern of immunochemical staining using a mouse anti-trout IgM monoclonal antibody.
Soybean agglutinin (SBA) was also employed as a leukoagglutinating reagent to study trout leucocytes. The recovered cells were in two main populations of agglutinated and unagglutinated cells. There was no significant difference in the response of the agglutinated or unagglutinated cells to the mitogens ConA or LPS, or in the staining patterns obtained using acid phosphatase or mouse anti-trout IgM monoclonal antibody.
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Findlay, C., Tatner, M.F. A comparative study of T and B lymphocytes in rainbow trout (Oncorhynchus mykiss) following their separation by nylon wool adherence and lectin agglutination techniques. Comp Haematol Int 4, 55–60 (1994). https://doi.org/10.1007/BF00368268
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DOI: https://doi.org/10.1007/BF00368268