Abstract
5-Aminolevulinic acid (ALA) was produced aerobically in the dark during growth on glucose by a newly isolated Chlorella sp. When levulinic acid (20 mM), a competitive inhibitor of ALA dehydratase, was added repeatedly to the medium, about 1.5 mM of ALA was produced extracellularly. Glutamate (30 mM) added with levulinic acid (20 mM, given repeatedly) enhanced ALA production up to 1.9 mM, indicating that ALA might be synthesized via the C-5 pathway.
References
BealeS.I. 1970 The biosynthesis of δ-aminolevulinic acid in Chlorella, Plant Physiology 45, 504–506.
EndoH., NakajimaK., ChinoR. & ShirotaM. 1974 Growth characteristics and cellular components of Chlorella regularis, heterotrophic fast growing strain. Agricultural and Biological Chemistry 38, 9–18.
RebeizC.A., JuvikJ.K. & RebeizC.C. 1988 Porphyric insecticides. I. Concept and phenomenology. Pesticide Biochemistry and Physiology 30, 11–27.
SasakiK., TanakaT., NishioN. & NagaiS. 1993 Effect of pH on the extracellular production of 5-aminolevulinic acid by Rhodobacter sphaeroides from volatile fatty acids. Biotechnology Letters 15, 859–864.
SasakiK., TanakaT., NishizawaY. & HayashiM. 1990 Production of herbicide, 5-aminolevulinic acid by Rhodobacter sphaeroides using the effluent of swine waste from an anaerobic digestor. Applied Microbiology and Biotechnology 32, 727–731.
Tanaka, T., Takahashi, K., Hotta, T., Takeuchi, Y., Konnai, M. 1992 Promotive effects of 5-aminolevulinic acid on yield of several crops. In Proceedings of the 19th Annual Meeting of Plant Growth Regulator Society of America, San Francisco, pp. 237–241. Washington DC: Plant Growth Regulator Society of America.
TanakaT., WatanabeK., HottaY., LinD., SasakiK. & NagaiS. 1991 Formation of 5-aminolevulinic acid under aerobic/dark condition by a mutant of Rhodobacter sphaeroides. Biotechnology Letters 13, 585–594.
Additional information
K. Sasaki was with the Hiroshima-Denki Institute of Technology, 6-20-1, Nakano, Akiku, Hiroshima, 739-03, Japan; and is now with the Department of Biotechnology. The University of New South Wales, Sydney, NSW, 2052, Australia; K. Watanabe, T. Tanaka and Y. Hotta are with the Cosmo Research Institute, 1134-2, Gongendo, Satte, Saitama, 340-01, Japan. S. Nagai is with the Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, 4-1, Kagamiyama 1 chome, Higashi-Hiroshima, 724. Japan.
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Sasaki, K., Watanabe, K., Tanaka, T. et al. 5-Aminolevulinic acid production by Chlorella sp. during heterotrophic cultivation in the dark. World Journal of Microbiology & Biotechnology 11, 361–362 (1995). https://doi.org/10.1007/BF00367123
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DOI: https://doi.org/10.1007/BF00367123