Abstract
H-2b-restricted cytolytic T lymphocytes (CTL) were generated against H-1, H-3, and H-4 antigens and tested against target cells of F1 hybrids between wild mice and inbred H-2 b mice. The congenic strain combinations for the CTL production were such that they tested one allele each at the H-1 and H-4 loci and four alleles at the H-3 locus. Most of the wild mice tested came from Southern Germany, but a few mice came from other European countries and Egypt and Israel. Virtually all wild mice typed as positive with CTL directed against H-3b and H-4b antigens; 32% of the F1 hybrids tested reacted with anti-H-1cCTL and 9% reacted with anti-H-3d CTL. The positive results were not caused by cross-reaction with allogeneic H-2 antigens controlled by the major histocompatibility complex (Mhc) genes of the wild mice. At least some of the H-3 and H-4 antigens detected by the CTL in the F1 hybrid were not identical with antigens of the immunizing strains. These results suggest a relatively low degree of polymorphism of the tested minor H loci in wild mice and further support the notion that minor H loci are unrelated to the Mhc.
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Adolph, S. and Klein, J.: Genetic variation of wild mouse populations in Southern Germany. I. Cytogenetic study. Genet. Res. (Camb.), in press, 1983
Bevan, M. J.: The major histocompatibility complex determines susceptibility to cytotoxic T cells directed against minor histocompatibility antigens. J. Exp. Med. 142: 1349–1364, 1975
Counce, S., Smith, P., Barth, R., and Snell, G. D.: Strong and weak histocompatibility gene differences in mice and their role in the rejection of homografts of tumors and skin. Ann. Surg. 144: 198–204, 1956
Duncan, W. R., Wakeland, E. K., and Klein, J.: Heterozygosity of H-2 loci in wild mice. Nature 281: 603–605, 1979
Figueroa, F., Zaleska-Rutczynska, Z., Adolph, S., Nadeau, J., and Klein, J.: Genetic variation of wild mouse populations in Southern Germany. II. Serological study. Genet. Res. (Camb.), in press, 1983
Gasser, D. L.: Genetic studies on the H-3 region of mice and implications for polymorphism of histocompatibility loci. Immunogenetics 3: 271–276, 1976
Gordon, R. D., Simpson, E., and Samelson, L. E.: In vitro cell mediated immune responses to the male specific (H-Y) antigen in mice. J. Exp. Med. 142: 1108–1120, 1975
Götze, D., Nadeau, J., Wakeland, E. K., Berry, R. J., Bonhomme, F., Egorov, K., Hjorth, J. P., Hoogstraal, H., Vives, J., Winking, H., and Klein, J.: Histocompatibility-2 system in wild mice. X. Frequencies of H-2 and Ia antigens in wild mice from Europe and Africa. J. Immunol. 124: 2675–2681, 1980
Graff, R. J.: Histocompatibility systems, except H-2: Mouse. In P. L. Altman and D. D. Katz (eds.): Inbred and Genetically Defined Strains of Laboratory Animals, part 1, pp. 119–122, Biological Handbooks III, Federation of American Societies for Experimental Biology, 1979
Graff, R. J. and Bailey, D. W.: The non-H-2 histocompatibility loci and their antigens. Transplant. Rev. 115: 26–49, 1973
Hauptfeld, V. and Klein, J.: A new histogenetic method for minor histocompatibility antigen typing. J. Immunol. 118: 423–426, 1977
Juretić, A., Nagy, Z. A., and Klein, J.: Detection of CML determinants associated with H-2 controlled E and E chains. Nature 289: 308–310, 1981
Klein, J.: Biology of the Mouse Histocompatibility-2 Complex. Springer Verlag, New York, 1975
Klein, J.: Allograft reaction: Atavism or immunological tour de force. In N. Le Douarin and A. Monroy (eds): Cell Lineage, Stem Cells and Cell Determinants, pp. 241–259, Elsevier-North Holland, Amsterdam, 1979
Klein, J. and Bailey, D. W.: Histocompatibility differences in wild mice: Further evidence for the existence of deme structure in natural populations of the house mouse. Genetics 68: 287–297, 1971
Klein, J. and Figueroa, F.: Polymorphism of the mouse H-2 loci. Immunol. Rev. 60: 23–57, 1981
Long, P. M., Lafuse, W. P., and David, C. S.: Serologic and biochemical identification of minor histocompatibility (H-4) antigens. J. Immunol. 127: 825–828, 1981
Nadeau, J. H., Wakeland, E. K., Götze, D., and Klein, J.: The population genetics of the H-2 polymorphism in European and North African populations of the house mouse (Mus musculus). Genet. Res. (Camb.) 37: 17–31, 1981
Nadeau, J. H., Collins, R. L., and Klein, J.: Organization and evolution of the mammalian genome: I. Polymorphism of H-2 linked loci. Genetics 102: 583–598, 1982
Rammensee, H.-G., and Klein, J.: Complexity ofthe histocompatibility-3 region in the mouse. J. Immunol. in press, 1983
Winn, H. J., Stevens, L. C., and Snell, G. D.: Tests for alternate methods for demonstrating in the H-1 isoantigen in mice. Transplant. Bull. 5: 18–21, 1958
Zink, G. L. and Heyner, S.: The production of non-H-2 histocompatibility alloantibodies in the mouse. Immunogenetics 4: 257–266, 1977
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Rammensee, HG., Klein, J. Polymorphism of minor histocompatibility genes in wild mice. Immunogenetics 17, 637–647 (1983). https://doi.org/10.1007/BF00366131
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DOI: https://doi.org/10.1007/BF00366131