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New HLA-A2 variants defined by monoclonal antibodies and cytotoxic T lymphocytes

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Abstract

Three HLA-A2 variants, A2-DW, A2-KC, and A2-Lee, were identified in three Chinese donors using a panel of monoclonal antibodies. A2-DW was negative with two of the ten HLA-A2 monoclonal antibodies tested, whereas A2-KC was negative with five of the ten and A-2 Lee was negative with one.

Epstein-Barr virus-specific cytotoxic T cells generated from the A2-DW donor recognized and killed target cells prepared from the A2-KC donor, but did not recognize target cells from HLA-A2.1, −A2.2, or −A2.4 donors. In isoelectric focusing studies, A2-DW and A2-KC focus in identical positions more acidic than the other HLA-A2 antigens tested.

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Authors and Affiliations

  1. Imperial Cancer Research Fund, Lincoln's Inn Fields, P.O. Box 123, WC2A 3PX, London, England

    Lorna J. Kennedy, J. Alejandro Madrigal & Julia G. Bodmer

  2. Department of Cancer Studies, The Medical School, Birmingham B15 2TJ, England

    Lesley E. Wallace & Alan B. Rickinson

Authors
  1. Lorna J. Kennedy
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  2. Lesley E. Wallace
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  3. J. Alejandro Madrigal
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  4. Alan B. Rickinson
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  5. Julia G. Bodmer
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Kennedy, L.J., Wallace, L.E., Madrigal, J.A. et al. New HLA-A2 variants defined by monoclonal antibodies and cytotoxic T lymphocytes. Immunogenetics 26, 155–160 (1987). https://doi.org/10.1007/BF00365905

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  • DOI: https://doi.org/10.1007/BF00365905

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