Summary
We have determined the base sequence of the restriction fragment Bam1-2 (3,593) of Paracentrotus lividus (sea urchin) mtDNA. This fragment contains, in addition to genes previously identified (part of the 12S rRNA, ND1 and part of the ND2 mRNA), a cluster of 15 tRNA genes located between the 12S and ND1 genes. Also to be found in the tRNA gene cluster, between the tRNAThr and tRNAPro genes, is a sequence of 134 bp which constitutes the only non-coding region of this DNA so far identified. The distinctive organization of the tRNA genes and the extreme size reduction of the non-coding region suggest the existence of unique mechanisms for the regulation of gene expression in this organism.
Similar content being viewed by others
References
Anderson S, Bankier A, Barrel BG, de Bruijn MHL, Coulson AR, Drouin J, Eperon IC, Nierlich DP, Roe BA, Sanger F, Schreier PH, Smith AJH, Staden R, Young IG (1981) Nature 280:457–465
Breitenberger CA, RajBhandary UL (1985) Trends Biochem Sci 10:478–483
Brown GG, Gadaleta G, Pepe G, Saccone C, Sbisà E (1986) J Mol Biol 192:503–512
Cantatore P, Saccone C (1987) Int Rev Cytol 108:149–208
Cantatore P, Roberti M, Rainaldi G, Morisco P, Gadaleta MN, Saccone C (1987a) Gene 53:41–54
Cantatore P, Roberti M, Rainaldi G, Gadaleta MN, Saccone C (1987b) In: Proceedings of Cold Spring Harbor Laboratory Meeting on Molecular Biology of Mitochondria and Chloroplast. Cold Spring Harbor New York, p 32
Chen EJ, Seeburg PH (1985) DNA 4:165–170
Ciliberto G, Raugei G, Costanzo F, Dente L, Cortese R (1983) Cell 32:725–733
Clary DO, Wolstenholme DR (1985) J Mol Evol 22:252–271
Cordell B, Swanstrom R, Goodman HH, Bishop JM (1979) J Biol Chem 254:1866–1874
de Bruijn MHL, Klug A (1983) EMBO J 2:1309–1321
Dujon B (1983) In: Schweyen RJ, Wolf K, Kaudewitz F (eds) Mitochondria 1983: nucleo-mitochondrial interactions. De Gruyter, Berlin, pp 1–24
Gouy M, Gautier C, Attimonelli M, Lanave C, Di Paola G (1985) CABIOS 1:167–172
Kim SH, Suddath FL, Quigley GJ, McPherson A, Sussman JL, Wang AHJ, Seeman NC, Rich A (1974) Science 185:435–440
Lagerkvist U (1978) Proc Natl Acad Sci USA 75:1759–1762
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor New York
Masukata H, Tomizawa J (1986) Cell 44:125–136
Roe BA, Ma D-P, Wilson RK, Wang JF-H (1985) J Biol Chem 260:9759–9774
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Wolstenholme DR, McFarlane JL, Okimoto R, Clary DO, Wahleithner JA (1987) Proc Natl Acad Sci USA 84:1324–1328
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cantatore, P., Roberti, M., Rainaldi, G. et al. Clustering of tRNA genes in Paracentrotus lividus mitochondrial DNA. Curr Genet 13, 91–96 (1988). https://doi.org/10.1007/BF00365762
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00365762