Skip to main content
SpringerLink
Log in

Visualization of chromosomes in mitotically arrested cells of the fission yeast Schizosaccharomyces pombe

  • Published:
Current Genetics Aims and scope Submit manuscript

Summary

Three sets of mitotic chromosomes were observed in wild type or cdc mutants (nda3-KM311 and nda2-KM52) of the fission yeast S. pombe by the DAPI staining method. The block of microtubular functions by thiabendazole or by the mutations caused their individual appearance in mitotically arrested cells. The chromosomes have a characteristic size; the length ratio of short, medium and long ones was roughly 1:2:3, consistent with the previous genetical data (Kohli et al. 1977). Double staining with ethidium bromide and DAPI showed that the nucleolus was always associated with the shortest chromosome. Pair-like structures resembling sister chromatids were also seen.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

cdc:

cell division cycle

DAPI:

4′,6-diamidino-2-phenylindole

kb:

kilo base pairs

nda:

nuclear division arrest

rRNA:

ribosomal RNA

References

  • Bak AL, Zeuthen J, Crick FHC (1977) Proc Natl Acad Sci USA 74:1595–1599

    Google Scholar 

  • Bostock CJ (1970) Expl Cell Res 60:16–26

    Google Scholar 

  • Davidse LC, Flach W (1977) J Cell Biol 72:174–194

    Google Scholar 

  • Gordon CN (1977) J Cell Sci 24:81–93

    Google Scholar 

  • Gutz H, Heslot H, Leupold U, Loprieno N (1974) Schizosaccharomyces. In: King, RC (ed) Handbook of Genetics. Plenum Press, New York, pp 395–446

    Google Scholar 

  • Kohli J, Hottinger H, Munz P, Strauss A, Thuriaux, P (1977) Genetics 87:471–489

    Google Scholar 

  • Leupold U (1950) Ctes Rend Lab Carlsberg Ser Physiol 24:381–480

    Google Scholar 

  • Leupold U (1958) Cold Spring Harb Symp Quant Biol 23:161–170

    Google Scholar 

  • Matsumoto S, Morikawa K, Yanagida M (1981) J Mol Biol 152:501–516

    Google Scholar 

  • McCully EK, Robinow DF (1971) J Cell Sci 9:475–507

    Google Scholar 

  • Nasmyth K, Nurse P (1981) Mol Gen Genet 182:119–124

    Google Scholar 

  • Robinow DF (1977) Genetics 87:491–497

    Google Scholar 

  • Tabata S (1981) Nucl Acid Res 9:6429–6437

    Google Scholar 

  • Toda T, Yamamoto M, Yanagida M (1981) J Cell Sci 52:271–287

    Google Scholar 

  • Toda T, Umesono K, Hirata A, Yanagida M (1983) J Mol Biol (submitted)

  • Umesono K, Toda T, Hayashi S, Yanagida M (1983) J Mol Biol (submitted)

  • Williamson DH, Fennell DJ (1975) In: Prescott DM (ed) Methods in Cell Biology, vol 12. Academic Press, New York, pp 335–351

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biophysics, Faculty of Science, Kyoto University, 606, Sakyo-ku, Kyoto, Japan

    K. Umesono, Y. Hiraoka, T. Toda & M. Yanagida

Authors
  1. K. Umesono
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Y. Hiraoka
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. Toda
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Yanagida
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Umesono, K., Hiraoka, Y., Toda, T. et al. Visualization of chromosomes in mitotically arrested cells of the fission yeast Schizosaccharomyces pombe . Current Genetics 7, 123–128 (1983). https://doi.org/10.1007/BF00365637

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00365637

Key words

Search

Navigation