Abstract
In the anuran Xenopus, as has been demonstrated previously in several species of urodele Amphibia, histone genes lie at the sphere organizer loci of the lampbrush chromosomes. They were located by in situ hybridization of a 3H-labelled histone H4 anti-sense cRNA probe applied to lampbrush preparations in which transcript RNA had been retained, and likewise to preparations in which transcripts were absent but whose DNA had been denatured prior to hybridization. In Xenopus the histone genes lie in intimate association with the spheres that are attached to the lampbrush chromosomes, but they are absent from spheres that lie free in the germinal vesicle. The Anura separated from the Urodela several hundred million years ago, so the sphere organizer/histone gene association is of great antiquity. This suggests that the association has a functional significance, though it is one that has yet to be discovered.
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References
Birnstiel ML, Busslinger M, Strub K (1985) Transcription termination and 3′ processing: the end is in site. Cell 41: 349–359
Bonner JJ, Pardue ML (1976) Ecdysone-stimulated RNA synthesis in imaginal discs of Drosophila melanogaster. Chromosoma 58: 87–99
Callan HG (1986) Lampbrush chromosomes. Springer, Berlin Heidelberg New York, pp 1–216
Callan HG, Gall JG (1991) Association of RNA with the B and C snurposomes of Xenopus oocyte nuclei. Chromosoma 101: 69–82
Callan HG, Gall JG, Berg CA (1987) The lampbrush chromosomes of Xenopus laevis: preparation, identification and distribution of 5S DNA sequences. Chromosoma 95: 236–250
Delobel N (1971) Étude descriptive des chromosomes en écouvillon chez Echinaster sepositus (Échinoderma, Astéride). Ann Embryol Morphogen 4: 383–396
Diaz MO, Gall JG (1985) Giant readthrough transcription units at the histone loci on lampbrush chromosomes of the newt Notophthalmus. Chromosoma 92: 243–253
Gall JG, Callan HG (1989) The sphere organelle contains small nuclear ribonucleoproteins. Proc Natl Acad Sci USA 86: 6635–6639
Gall JG, Stephenson EC, Erba HP, Diaz MO, Barsacchi-Pilone G (1981) Histone genes are located at the sphere loci of newt lampbrush chromosomes. Chromosoma 84: 159–171
Hentschell CC, Birnstiel ML (1981) The organization and expression of histone gene families. Cell 25: 301–313
Hilder VA, Livesey RN, Turner PC, Vlad MT (1981) Histone gene number in relation to C-value in amphibians. Nucleic Acids Res 9: 5737–5746
Jacob E, Malacinski G, Birnstiel ML (1976) Reiteration frequency of the histone genes in the genome of the amphibian Xenopus laevis. Eur J Biochem 69: 45–54
Old RW, Woodland HR, Ballantine JEM, Aldridge TC, Newton CA, Bains WA, Turner PC (1982) Organization and expression of cloned histone gene clusters from Xenopus laevis and X. borealis. Nucleic Acids Res 23: 7561–7580
Perry M, Thomsen GH, Roeder RG (1985) Genomic organization and nucleotide sequence of two distinct histone gene clusters from Xenopus laevis. Identification of novel conserved upstream sequence elements. J Mol Biol 185: 479–499
Porter KR (1972) Herpetology. Saunders, Philadelphia
Stephenson EC, Erba HP, Gall JG (1981) Histone gene clusters of the newt Notophthalmus are separated by long tracts of satellite DNA. Cell 24: 639–647
van Dongen WMAM, de Laaf L, Zaal R, Moorman AFM, Destrée OHJ (1981) The organization of the histone genes in the genome of Xenopus laevis. Nucleic Acids Res 9: 2297–2311
van Dongen WMAM, Moorman AFM, Destrée OHJ (1984) DNA organization and expression of Xenopus histone genes. In: Stein GS, Stein JL, Marzluff WF (eds) Histone genes: structure, organization and regulation. Wiley, New York, pp 199–224
Wu Z, Murphy C, Callan HG, Gall JG (1991) Small nuclear ribonucleoproteins and heterogeneous nuclear ribonucleoproteins in the amphibian germinal vesisicle: loops, spheres, and snurposomes. J Cell Biol 113: 465–483
Zernik M, Heintz N, Biome I, Roeder RG (1980) Xenopus laevis histone genes: variant H1 genes are present in different clusters. Cell 22: 807–815
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Callan, H.G., Gall, J.G. & Murphy, C. Histone genes are located at the sphere loci of Xenopus lampbrush chromosomes. Chromosoma 101, 245–251 (1991). https://doi.org/10.1007/BF00365156
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DOI: https://doi.org/10.1007/BF00365156