Abstract
Two-dimensional chymotryptic peptide maps of Lyt-1.1 and Lyt-1.2 from Lyt-1 congenic thymocytes labeled with 125I in the usual way before lysis did not significantly differ, but there was a characteristic difference between maps of Lyt-1.1 and Lyt-1.2 obtained from 125I-labeled solubilized membrane fragments. We conclude that the Lyt-1 locus of chromosome 19 includes the protein-structural gene for Lyt-1. This conclusion is further supported by evidence with 35S-cysteine-labeled thymocytes: Thus an early 62K intermediate form, and a 60K form from tunicamycin-treated cells devoid of N-linked and O-linked carbohydrates, were precipitated by Lyt-1 alloantibody, which implies that the allo-specificity of Lyt-1 glycoprotein (67K) resides partly or wholly in protein.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Boyse, E. A., Miyazawa, M., Aoki, T., and Old, L. J.: Ly-A and Ly-B: two systems of lymphocyte isoantigens in the mouse. Proc. Roy. Soc. Lond. 170: 174–193, 1968
Cantor, H. and Boyse, E. A.: Lymphocytes as models for the study of mammalian cellular differentiation. Immunol. Rev. 33: 105–124, 1977
Durda, P. J., Shapiro, C., and Gottlieb, P. D.: Partial molecular characterization of the Lyt-1 alloantigen on mouse thymocytes. J. Immunol. 120: 53–57, 1978
Elder, J. H., Gautoch, J. W., Jensen, F. C., Lerner, R. A., Hartley, J. W., and Rowe, W. P.: Biochemical evidence that MCF murine leukemia virsus are envelope (env) gene recombinants. Proc. Natl. Acad. Sci. U.S.A. 74: 4676–4680, 1977
Itakura, K., Hutton, J. J., Boyse, E. A., and Old, L. J.: Linkage groups of the theta and Ly-A loci. Nature 230: 126–127, 1971
Jett, M., Seed, T. M., and Jamieson, G. A.: Isolation and characterization of plasma membranes and intact nuclei from lymphoid cells. J. Biol. Chem. 252: 2134–2142, 1977
Kimura, S., Tada, N., and Hämmerling, U.: A new lymphocyte alloantigen (Ly-10) controlled by a gene linked to the Lyt-1 locus. Immunogenetics 10: 363–372, 1980
Kimura, S., Tada, N., Liu-Lam, Y., and Hämmerling, U.: Further genetic characterization of mouse Ly-m10 alloantigen. Immunogenetics 17: 211–214, 1983
Laemmli, U.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685, 1970
Ledbetter, J. A., Evans, R. L., Lipinski, M., Cunningham-Rundles, C., Good, R. A., and Herzenberg, L. A.: Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J. Exp. Med. 153: 310–323, 1981
Pecoud, A. R. and Conrad, D. H.: Characterization of the IgE receptor by tryptic mapping. J. Immunol. 127: 2208–2214, 1981
Rothenberg, E. and Boyse, E. A.: Synthesis and processing of molecules bearing thymus leukemia antigen. J. Exp. Med. 150: 777–791, 1979
Shen, F. W.: Monoclonal antibodies to mouse lymphocyte differentiation antigens. In G. L. Hammerling, U. Hammerling, and J. F. Kearney (eds.): Monoclonal Antibodies and T-Cell Hybridomas: Perspectives and Technical Advances, pp. 25–31, Elsevier/North-Holland Biomedical Press, Amsterdam, 1981
Tung, J. S., Scheid, M. P., Pierotti, M. A., Hammerling, U., and Boyse, E. A.: Structural features and selective expression of three Ly-5+ cell-surface molecules. Immunogenetics 14: 101–106, 1981
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tung, JS., Shen, FW., Deere, M.C. et al. Structure and biosynthesis of Lyt-1. Immunogenetics 20, 125–130 (1984). https://doi.org/10.1007/BF00364484
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00364484