Abstract
A mouse cDNA library derived from the EL4 cell line (b-haplotype) was screened with a probe containing a small part of the H-2Kb coding region. One of the clones isolated, pH203, encodes a protein whose deduced amino acid sequence is identical with the known sequence of H-2Db in 141 of 141 positions available for comparison. The clone, therefore, is believed to code for the H-2Db transplantation antigen. The cDNA insert of pH203 contains the coding region for residues 82 through the carboxy-terminus of H-2Db, and includes 476 nucleotides of the 3′-untranslated sequence. Comparison between the H-2Db cDNA clone and a previously isolated H-2Kb cDNA clone shows homologies of 83% and 91% at the amino acid and nucleotide levels, respectively. Analysis of DNA sequences at the 3′-coding and untranslated regions suggests that the mRNAs of H-2Kb and H-2Db are spliced differently at their 3′-coding ends.
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References
Bodmer, W. F.: A new genetic model for allelism at histocompatibility and other complex loci: Polymorphism for control of gene expression. Transplant. Proc 4: 1471–1475, 1973
Coligan, J. E., Kindt, T. J., Nairn, R., Nathenson, S. G., Sachs, D. H., and Hansen, T. H.: Primary structural studies of an H-2L molecule confirms that it is a unique gene product with homology to H-2K and H-2D antigens. Immunology 77: 1134–1138, 1980
Coligan, J. E., Kindt, T. J., Uehara, H., Martinko, J., and Nathenson, S. G.: Primary structure of a murine transplantation antigen. Nature 291: 35–39, 1981
Demant, P and Iványi, D.: Further molecular complexities of H-2 K- and D-region antigens. Nature 290: 146–149, 1981
Hansen, T. H. and Sachs, D. H.: Isolation and antigenic characterization of the product of a third polymorphic H-2 locus, H-2L. J. Immunology 121: 1469–1472, 1978
Hansen, T. H., Ozato, K., Melino, M. R., Coligan, J. E., Kindt, T. J., Jandinski, J. J., and Sachs, D. H.: Immunochemical evidence in two haplotypes for at least three D region-encoded molecules, D, L, and R. J. Immunol. 126: 1713–1716, 1981
Kimball, E. S., Maloy, W. L., Martinko, J. M., Nathenson, S. G., and Coligan, J. E.: Structional studies on H-2Db molecules indicate that murine major histocompatibility b and d haplotype alloantigens share structural features. Mol. Immunol. 17: 1283–1291, 1980
Kimball, E. S., Maloy, W. L., and Coligan, J. E.: Evidence for three carbohydrate prosthetic groups on mouse histocompatibility antigens H-2Kd and H-213b. Mol. Immunology 18: 677–689, 1981
Klein, J.: Biology of the Mouse Histocompatibility-2 Complex, Springer-Verlag, New York, 1975
Klein, J., Juretic, A., Baxevanis, C. N., and Nagy, Z. A.: The traditional and a new version of the mouse H-2 complex. Nature 291: 455–460, 1981
Levy, R. B. and Hansen, T. H.: Functional studies of the products of the H-2L locus. Immunogenetics 10: 7–17, 1980
Maloy, W. L., Nathenson, S. G., and Coligan, J. E.: Primary structure of murine major histocompatibility complex alloantigens. J. Biol. Chem. 256: 2863–2872, 1981
Maloy, W. L. and Coligan, J. E.: Primary structure of the H-2Db alloantigen. II. Additional amino acid sequence information, localization of a third site of glycosylation, and evidence for K and D region specific sequences. Immunogenetics 15: 11–22, 1982
Marshall, R. D.: Glycoproteins. Ann. Rev. Biochem. 41: 673–702, 1972
Maxam, A. and Gilbert, W.: Sequencing end-labeled DNA with basespecific chemical cleavages. In Methods in Enzymology 65: 499–560, 1980
Michaelson, J., Flaherty, L., Vitteta, E., and Poulik, M. D.: Molecular similarities between the Qa-2 alloantigen and other gene products of the 17th chromosome of the mouse. J. Exp. Med. 145: 1066–1070, 1977
Nairn, R., Nathenson, S. G., and Coligan, J. E.: Isolation, characterization and amino acid sequence studies of the cyanogene bromide fragments of the H-2Dd glycoprotein. Eur. J. Immunol 10: 495–503, 1980
Nairn, R., Nathenson, S. G., and Coligan, J. E.: Amino acid sequence of cyanogen bromide fragment CN-C (residues 29–48) of the mouse histocompatibility antigen H-2Dd: A comparison of the amino-terminal 100 residues of H-2Dd, Db, Kd and Kb reveals discrete areas of diversity. Biochemistry 20: 4739–4745, 1981
Nathenson, S. G., Uchara, H., Ewenstein, B. M., Kindt, T. J., and Coligan, J. E.: Primary structural analysis of the transplantation antigens of the murine H-2 major histocompatibility complex. Ann. Rev. Biochem. 50: 1025–1052, 1981
Orr, H. T., Lancet, D., Robb, R. J., Lopez de Castro, J. A., and Strominger, J. L.: The heavy chain of human histocompatibility antigen HLA-B7 contains an immunoglobulin region. Nature 282: 266–270, 1979
Proudfoot, N. J. and Brownlee, G. G.: 3′ Non-coding region sequences in eukaryotic messenger RNA. Nature 263: 211–214, 1976
Reyes, A. A., Johnson, M. J., Schöld, M., Ito, H., Ike, Y., Morin, C., Itakura, K., and Wallace, R. B.: Identification of an H-2Kb-related molecule by molecular cloning. Immunogenetics 14: 383–392, 1981
Reyes, A. A., Schöld, M., Itakura, K., and Wallace, R. B.: Isolation of a cDNA clone for the murine transplantation antigen H-2Kb. Proc. Natl. Acad. Sci. U.S.A.: in press, 1982
Silver, J and Hood, L.: Preliminary amino acid sequences of transplantation antigens: Genetic and evolutionary implications. Contemp. Top. Mol. Immunol. 5: 35–86, 1976
Soloski, M. J., Uhr, J. W., Flaherty, L., and Vitetta, E. S.: Qa-2, H-2K, and H-2D alloantigens evolved from a common ancestral gene. J. Exp. Med. 153: 1080–1093, 1981
Stanton, T. H. and Hood, L.: Biochemical identification of the Qa-1 alloantigen. Immunogenetics 11: 309–314, 1980
Steinmetz, M., Moore, K. W., Frelinger, J. G., Sher, B. T., Shen, F.-W., Boyse, E. A., and Hood, L.: A pseudogene homologous to mouse transplantation antigens: Transplantation antigens are encoded by eight exons that correlate with protein domains. Cell 25: 683–692, 1981a
Steinmetz, M., Frelinger, J. G., Fisher, D., Hunkapiller, T., Pereira, D., Weissman, S. M., Uehara, H., Nathenson, S., and Hood, L.: Three cDNA clones encoding mouse transplantation antigens: homology to immunoglobulin genes. Cell 24: 125–134, 1981b
Strominger, J. L., Orr, H. T., Parham, P., Ploegh, H. L., Mann, D. L., Bilofsky, H., Saroff, H. A., Wu, T. T., and Kabat, E. A.: An evaluation of the significance of amino acid sequence homologies in human histocompatibility antigens (HLA-A and HLA-B) with immunoglobulins and other proteins, using relatively short sequences. Scand. J. Immunol. 11: 573–592, 1980
Uehara, H., Ewenstein, B. M., Martinko, J. M., Nathenson, S. G., Coligan, J. E., and Kindt, T. J.: Primary structure of murine major histocompatibility complex alloantigens: amino acid sequence of the amino-terminal one hundred and seventy-three residues of the H-2Kb glycoprotein. Biochemistry 19: 306–315, 1980a
Uehara, H., Ewenstein, B. M., Martinko, J. M., Nathenson, S. G., Kindt, T. J., and Coligan, J. E.: Primary structure of murine major histocompatibility alloantigens: amino acid sequence of the cyanogen bromide fragment Ia (Positions 139–228) from the H-2Kb molecule. Biochemistry 19: 6182–6188, 1980b
Vitteta, E. S., Poulik, M. D., Klein, J., and Uhr, J. W.: Beta 2-microglobulin is selectively associated with H-2 and TL alloantigens on murine lymphoid cells. J. Exp. Med. 144: 179–192, 1976
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Reyes, A.A., Schöld, M. & Wallace, R.B. The complete amino acid sequence of the murine transplantation antigen H-2Db as deduced by molecular cloning. Immunogenetics 16, 1–9 (1982). https://doi.org/10.1007/BF00364437
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DOI: https://doi.org/10.1007/BF00364437