Cytochemical observations concerning the formation, release, and transport of lipid secretory products in the interstitial (thecal) cells of the rabbit ovary

Summary

The release of lipid droplets from the ovarian interstitial gland cells of thecal origin into the circulating blood has been studied by cytochemical methods. Estrous, preovulatory, and postovulatory ovaries of rabbits were used. Ovulation was induced by human chorionic gonadotropin (HCG) administration. The cytoplasm in the gland cells of estrous ovaries is filled with lipid droplets consisting of cholesterol or its esters, triglycerides, and some phospholipids. Mitochondria, Golgi zone, and diffuse lipoproteins are well differentiated. The lipid droplets decrease in interstitial gland cells of preovulatory ovaries stimulated with HCG. The release of lipid droplets by the formation of vacuoles is closely accompanied by a considerable loss of cytoplasm and its organelles; consequently the cells are reduced in size. Degenerating gland cells do not release their lipids because they are refractory to gonadotropic stimulation. The released secretory products are found in the form of discrete bodies between gland cells, in regions adjacent to blood vessels, and in the lumen of the latter. The mechanism by which the liberated lipids are transported into the circulation could not be determined. The depletion of lipid droplets is clearly related with the production of 20a-OH steroid (20a-hydroxy-pregn-4-en-3-one) and progesterone studied by other workers.

The replenishment of cytoplasm and its organelles, and the formation and storage of secretory lipid granules have also been studied both in the preovulatory and postovulatory ovaries. The organelles could not be seen to be visibly related to lipid synthesis.