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Chromosomal banding patterns and karyotype evolution in three pig kidney cell strains (PK15, F and RP)

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Abstract

Some of the techniques used to obtain banding patterns in human karyotype are adapted here to three pig kidney cell strains (PK15, F and RP). These strains were established respectively in 1955, 1962 and 1969. The banding techniques used are: controlled heating, ASG technique, alkaline treatment and proteolytic digestion with trypsin or pronase. Knowing the specific banding of the pig karyotype, it has been possible to study the chromosomal rearrangements observed in the heteroploid cell strains. If the strain is old, the rearrangements are more numerous. However, they are the same as the ones usually described: in the three strains, one of the two chromosomes of each pair is retained unchanged as judged by its banding. The other chromosome is either present, lost or modified. It may constitute part of a marker chromosome.

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  1. Laboratoire de Génétique Cellulaire, I.N.R.A., Toulouse

    Geneviève Echard

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  1. Geneviève Echard
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Echard, G. Chromosomal banding patterns and karyotype evolution in three pig kidney cell strains (PK15, F and RP). Chromosoma 45, 133–149 (1974). https://doi.org/10.1007/BF00362307

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