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Natural oils are better than organic solvents for the conversion of soybean sterols to 17-ketosteroids by Mycobacterium fortuitum

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Abstract

Conversion of soybean sterols to 17-ketosteroids by thawed cells of Mycobacterium fortuitum is strongly inhibited by organic solvents. Use of natural oils instead of the solvents leads to enhanced activity, even after 4 h.

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References

  • Arinbasarova, A.Y. & Koshcheenko, K.A. 1983 Use of water-organic systems in enzymic steroid transformation. Prikladnaya Biokhimiya i Mikrobiologiya 19, 20–32.

    Google Scholar 

  • Ceen, E.G., Herrmann, J.P.R. & Dunnill, P. 1987 Solvent damage during free cell catalysis and its avoidance: studies of 11α-hydroxylation of progesterone by Aspergillus ochraceus. Enzyme and Microbial Technology 9, 365–368.

    Google Scholar 

  • Flygare, S. & Larsson, P.O. 1989 Steroid transformation in aqueous two-phase systems: side chain degradation of cholesterol by Mycobacterium sp. Enzyme and Microbial Technology 11, 752–759.

    Google Scholar 

  • Pinheiro, H.M. & Cabral, J.M.S. 1991 Effects of solvent molecular toxicity and microenvironment composition on the 229-1 activity of Arthrobacter simplex cells. Biotechnology and Bioengineering 37, 97–102.

    Google Scholar 

  • Pontius, D.J. 1962 Spectrophotometric determination of androsterones by a modified pyridine procedure. Analytical Chemistry 34, 168–169.

    Google Scholar 

  • Smolders, A.J.J., Pinheiro, H.M., Noronha, P. & Cabral, J.M.S. 1991 Steroid bioconversion in a microemulsion system. Biotechnology and Bioengineering 38, 1210–1217.

    Google Scholar 

  • Stadtman, T.C. 1957 Preparation and assay of cholesterol and ergosterol. Methods in Enzymology 3, 392–394.

    Google Scholar 

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The authors are with the School of Life Sciences, Devi Ahilya Vishwavidyalaya, Vigyan Bhawan, Khandwa Road Campus, Indore-452 001, India.

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Phase, N., Patil, S. Natural oils are better than organic solvents for the conversion of soybean sterols to 17-ketosteroids by Mycobacterium fortuitum . World Journal of Microbiology & Biotechnology 10, 228–229 (1994). https://doi.org/10.1007/BF00360894

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  • DOI: https://doi.org/10.1007/BF00360894

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