Abstract
Sixteen polymorphic markers, including markers for eight new loci, forming three linkage groups, were assigned to rat Chromosome (Chr) 4 by linkage analysis of the progeny of an F2 intercross of Fischer (F344/N) and Lewis (LEW/N) inbred rats. One gene, Igk, was mapped by restriction fragment length polymorphism (RFLP) analysis. One marker for Tcrb was identified by the polymorphic insertion of a repetitive LINE element. The remaining 14 markers contained polymorphic simple sequence repeats (SSRs). Ten were identified in genes (Tgfa, Npy, Prss1, Prss2, Aldr1, Iapp, Prp, Eno2, Cacnlla1, and Il6), one was identified in a sequence related to a gene (Egr4l1), and three were identified in anonymous DNA segments. The SSR markers were highly polymorphic in 16 inbred rat strains. These markers expand the genetic map of the rat and should be useful in future genetic studies of inbred rats.
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References
Blankenhorn, E.P., Smith, P.D., Williams, C.B., Gutman, G.A. (1992). Alleles of the rat T-cell receptor beta chain gene complex. Immunogenetics 35, 324–331.
GBASE (1994). The genomic database of the mouse maintained at The Jackson Laboratory by D.P. Doolittle, A.L. Hillyard, L.J. Maltais, J.N. Guidi, M.T. Davisson, and T.H. Roderick.
GDB (1994). The Genome Data Base of the Welch Medical Library. (Baltimore: Johns Hopkins University.)
Goldmuntz, E.A., Remmers, E.F., Zha, H., Mathern, P., Du, Y., Crofford, L.J., Wilder, R.L. (1993). Genetic map of seven polymorphic markers comprising a single linkage group on rat Chr 5. Mamm. Genome 4, 670–675.
Green, E. (1981). Linkage, recombination and mapping. In Genetics and Probability in Animal Breeding Experiments, E. Green, ed. (New York: Macmillan), pp. 77–113.
Hilbert, P., Lindpaintner, K., Beckmann, J.S., Serikawa, T., Soubrier, F., Dubay, C., Cartwright, P., De Gouyon, B., Julier, C., Takahasi, S., Vincent, M., Ganten, D., Georges, M., Lathrop, G.M. (1991). Chromosomal mapping of two genetic loci associated with blood-pressure regulation in hereditary hypertensive rats. Nature 353, 521–529.
Jacob, H.J., Petterson, A., Wilson, D., Mao, Y., Lernmark, A., Lander, E.S. (1992). Genetic dissection of autoimmune type I diabetes in the BB rat. Nature Genet. 2, 56–60.
Jacob, H.J., Brown, D.M., Bunker, R.K., Daly, M.J., Dzau, V.J., Goodman, A., Koike, G., Kren, V., Kurtz, T., Lernmark, A., Levan, G., Mao, Y., Pettersson, A., Pravenec, M., Simon, J.S., Szpirer, C., Szpirer, J., Trolliet, M.R., Winer, E.S., Lander, E.S. (1995) A genetic linkage map of the laboratory rat, Rattus norvegicus. Nature Genet. 9:63–69.
Klinga-Levan, K., Szpirer, J., Levan, G., Szpirer, C. (1993). The rat map 1993. Rat News Lett. 29, 16–48.
Lander, E.S., Green, P., Abrahamson, J., Barlow, A., Daly, M.J., Lincoln, S.E., Newburg, L. (1987). MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1, 174–181.
Oerter, K.E., Munson, P.J., McBride, W.O., Rodbard, D. (1990). Computerized estimation of size of nucleic acid fragments using the fourparameter logistic model. Anal. Biochem. 189, 235–243.
Remmers, E.F., Goldmuntz, E.A., Cash, J.M., Crofford, L.J., Misiewicz-Poltorak, B., Zha, H., Wilder, R.L. (1992). Genetic map of 9 polymorphic loci comprising a single linkage group on rat chromosome 10: evidence for linkage conservation with human chromosome 17 and mouse chromosome 11. Genomics 14, 618–623.
Remmers, E.F., Goldmuntz, E.A., Zha, H., Crofford, L.J., Cash, J.M., Mathern, P., Du, Y., Wilder, R.L. (1993). Linkage map of seven polymorphic markers on rat Chromosome 18. Mamm. Genome 4, 265–270.
Serikawa, T., Kuramoto, T., Hilbert, P., Mori, M., Yamada, J., Dubay, C.J., Lindpaintner, K., Ganten, G., Guénet, J.-L., Lathrop, G.M., Beckmann, J.S. (1992). Rat gene mapping using PCR-analyzed microsatellites. Genetics 131, 701–721.
Yamada, J., Kuramoto, T., Serikawa, T. (1994). A rat genetic linkage map and comparative maps for mouse or human homologous rat genes. Mamm. Genome 5, 63–83.
Zha, H., Wilder, R.L., Goldmuntz, E.A., Cash, J.M., Crofford, L.J., Mathern, P., Remmers, E.F. (1993). Linkage map of 10 polymorphic markers on rat chromosome 2. Cytogenet. Cell Genet. 63, 117–122.
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Goldmuntz, E.A., Remmers, E.F., Du, Y. et al. Genetic map of 16 polymorphic markers forming three linkage groups assigned to rat Chromosome 4. Mammalian Genome 6, 459–463 (1995). https://doi.org/10.1007/BF00360654
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DOI: https://doi.org/10.1007/BF00360654