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Effects of deuterium oxide and temperature on heart rate in Drosophila melanogaster

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Summary

A non-intrusive optical technique has been developed to monitor heartbeat in late third-instar Drosophila larvae. Heartbeat in this insect is an oscillation that is not temperature compensated. Deuterium oxide lengthens the period of a number of high and low frequency oscillators and clocks in a variety of organisms. To determine whether deuterium affects heart rate, flies were raised on proteated and deuterated media and their heartbeat was monitored at four temperatures ranging from 18 to 33°C. The rate of heartbeat increased linearly with increasing temperature, and decreased with increasing concentrations of deuterium. There was a significant interaction between temperature and deuterium: the higher the concentration of deuterium oxide the less temperature-sensitive was the heart rate. Raising temperatures also increased the amount of “noise” in the rhythm: signal-to-noise ratio, which characterizes the amount of power in a rhythmic signal, decreased with increasing temperatures. Deuterium oxide had no effect on signal-to-noise ratio.

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Abbreviations

f :

frequency

HR:

heart rate

MESA:

maximum entropy spectral analysis

SNR:

signal-to-noise ratio

T :

temperature

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Authors and Affiliations

  1. Department of Zoology, University of Maine, 04469, Orono, ME, USA

    Lori A. White, John M. Ringo & Harold B. Dowse

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  1. Lori A. White
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  2. John M. Ringo
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  3. Harold B. Dowse
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White, L.A., Ringo, J.M. & Dowse, H.B. Effects of deuterium oxide and temperature on heart rate in Drosophila melanogaster . J Comp Physiol B 162, 278–283 (1992). https://doi.org/10.1007/BF00357535

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  • DOI: https://doi.org/10.1007/BF00357535

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