Skip to main content
SpringerLink
Log in

Binding of puromycin to E. coli ribosomes. Effects of puromycin analogues and peptide bond formation inhibitors

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

3H-puromycin binds to bacterial ribosomes, in the presence of ethanol, under the experimental conditions of the fragment reaction assay. The binding is feeble, takes place at 0°C, is partially inhibited by chloramphenicol and lincomycin and totally by sparsomycin. 3H-puromycin binding is hardly affected by the 3′ aminonucleoside of puromycin, is well inhibited by the L-Phenylalanine and L-Leucine analogues of puromycin and totally blocked by L-Phenylalanyl-adenosine.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Monro, R. E., Staehelin, T., Celma, M. L., and Vazquez, D., Cold Spring Harbor Symp. Quant. Biol. 34, 357 (1969).

    Google Scholar 

  2. Vazquez, D., Battaner, E., Neth, R., Heller, G., and Monro, R. E., Cold Spring Harbor Symp. Quant. Biol. 34, 369 (1969).

    Google Scholar 

  3. Nathans, D. and Neidle, A., Nature 197, 1076 (1963).

    Google Scholar 

  4. Harbon, S. and Chapeville, F., Eur. J. Biochem. 13, 375 (1970).

    Google Scholar 

  5. Rychlík, I., Černá, J., Chládek, S., Pulkrábek, P., and Zemlička, J., Eur. J. Biochem. 16, 136 (1970).

    Google Scholar 

  6. Harris, R. J., Hanlon, J. E., and Symons, R. H., Biochim. Biophys. Acta 240, 244 (1971).

    Google Scholar 

  7. Celma, M. L., Monro, R. E., and Vazquez, D., FEBS Letters 13, 247 (1971).

    Google Scholar 

  8. Hishizawa, T. and Pestka, S., Arch. Biochem. Biophys. 147, 624 (1971).

    Google Scholar 

  9. Celma, M. L., Monro, R. E., and Vazquez, D., FEBS Letters 6, 273 (1970).

    Google Scholar 

  10. Cabrer, B., Vazquez, D., and Modolell, J., Proc. Nat. Acad. Sci. U.S. 69, 733 (1972).

    Google Scholar 

  11. Fernandez-Muñoz, R., Monro, R. E., Torres-Pinedo, R., and Vazquez, D., Eur. J. Biochem. 23, 185 (1971).

    Google Scholar 

  12. Vazquez, D., Biochim. Biophys. Acta 144, 277 (1966).

    Google Scholar 

  13. Fernandez-Muñoz, R., Monro, R. E., and Vazquez, D., Methods Enzymol. 20, 446 (1971).

    Google Scholar 

  14. Goldberg, I. H. and Mitsugi, K., Biochemistry 6, 382 (1967).

    Google Scholar 

  15. Černá, J., Jonak, J., and Rychlík, I., Biochim. Biophys. Acta 240, 109 (1971).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Instituto de Biología Celular, Madrid, Spain

    Rafael Fernandez-Muñoz & David Vazquez

Authors
  1. Rafael Fernandez-Muñoz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. David Vazquez
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fernandez-Muñoz, R., Vazquez, D. Binding of puromycin to E. coli ribosomes. Effects of puromycin analogues and peptide bond formation inhibitors. Molecular Biology Reports 1, 27–32 (1973). https://doi.org/10.1007/BF00357402

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00357402

Keywords

Search

Navigation