Abstract
A peptidic effector from calf thymus causes a strong stabilization of DNA doublestranded molecule in vitro. The active factor was isolated from aqueous ultrafiltered thymus extracts and purified by means of chromatography on DEAE-cellulose and then on Dowex 50 WX2. The purified thymic factor was characterized as a peptide of low molecular weight (<5000). The biological activity of the thymic factor cannot be attributed to a histone fragment. Melting data of the control DNA and of the DNA-active factor complex in various conditions of ionic strength and dielectric constant of the solution medium are recorded.
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Gianfranceschi, G.L., Amici, D. & Guglielmi, L. Stabilization of double-stranded DNA molecule by nonhistone peptidic effector from calf thymus. Molecular Biology Reports 3, 55–64 (1976). https://doi.org/10.1007/BF00357209
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DOI: https://doi.org/10.1007/BF00357209