Abstract
The molecular weights of the predominant rRNA precursors as well as those of 26-S and 17-S mature rRNA from Saccharomyces carlsbergensis were determined by polyacrylamide gel electrophoresis in the presence of formamide. Mature 26-S+5.8-S rRNA was found to have a molecular weight of 1.24×106 while their immediate precursor, 29-S RNA, had a molecular weight of 1.52×106. Values of 0.70×106 and 0.82×106 were obtained for the molecular weights of mature 17-S rRNA and its 18-S precursor. Finally the 37-S precursor, common to both 29-S and 18-S RNA, was found to have a molecular weight of 2.80×106. Each precursor rRNA, therefore, contains extra sequences not found at the next stage of maturation.
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Brand, R.C., Planta, R.J. The molecular weights of yeast ribosomal precursor RNAs. Molecular Biology Reports 2, 321–325 (1975). https://doi.org/10.1007/BF00357019
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DOI: https://doi.org/10.1007/BF00357019