Summary
Whole chromosomes from the fungal phytopathogen Leptosphaeria maculans were separated by transverse alternating field electrophoresis. The chromosome complements from several isolates of both highly and weakly virulent strains were compared. Small variations in chromosome size and apparent number were detected among isolates of the same strain. However, dramatic differences in both chromosome number and size were found when isolates of the highly virulen strain were compared to those of the weakly virulent. Highly virulent isolates had 6–8 distinct bands whereas weakly virulent isolates had 12–14. The genome sizes were estimated to be at least 8.6x106 base pairs for the virulent strain and 1.6x107 base pairs for the weakly virulent strain. The major differences found in the chromosome complements of the two strains, in combination with results of other biochemical, morphological, and genetic studies, indicate that they are distinct species.
Similar content being viewed by others
References
Bonman JM, Gabrielson RL, Williams PH, Delwiche PA (1981) Plant Dis 65: 865–867
Brody H, Carbon J (1989) Proc Natl Acad Sci USA 86: 6260–6263
Carle GF, Olson MV (1985) Proc Natl Acad Sci USA 82: 3756–3760
Cunningham GH (1927) N Z Dept Agri Bull, 133
Delwiche PA (1980) Ph.D. Thesis, University of Wisconsin, Madison
DeMarch G, Séguin-Swartz G, Petrie GA (1986) Can J Plant Pathol 8: 422–428
Gardiner K. Laas W, Patterson D (1986) Somat Cell Mol Gen 12: 185–195
Gugel RK, Séguin-Swartz G, Petrie GA (1990) Can J Plant Pathol 12: 75–82
Hill CB, Hua XX, Williams PH (1984) Cruciferae Newslett 9: 79
Koch E, Badawy HMA, Hoppe HH (1989) J Phytopathol 124: 52–62
Lacoste L (1965) Thèse de Doctorat des Sciences, Toulouse
Magee BB, Magee PT (1987) J Gen Microbiol 133: 425–430
Masel A, Braithwaite K, Irwin J, Manners J (1990) Curr Genet 18: 81–86
McGee DC, Petrie GA (1978) Phytopathology 68: 625–630
Newman PL (1984) Plant Pathol 33: 205–210
Orbach MJ, Vollrath D, Davis RW, Yanofsky C (1988) Mol Cell Biol 8: 1469–1473
Pedras MSC, Séguin-Swartz G, Abrams SR (1990) Phytochemistry 29: 777–782
Petrie GA, Mortensen K, Dueck J (1985) Can Plant Dis Surv 65: 35–41
Petrie GA (1988) Can J Plant Pathol 10: 188–190
Polacheck I, Lebens GA (1989) J Gen Microbiol 135: 65–71
Punithalingham F, Holliday P (1972) CMI descriptions of pathogenic fungi and bacteria no 331. Commonwealth Agric Bur, 34
Rollo F, Ferracuti T, Pacilli A (1989) Curr Genet 16: 477–479
Schwartz DC, Saffran W, Welsh J, Haas R, Goldenberg M, Cantor CR (1982) Cold Spring Harbor Symp Quant Biol 47: 189–195
Specht CA, Munoz-Rivas A, Novotny CP, Ullrich RC (1988) Exp Mycol 12: 357–366
Stewart G, Furst A, Avdalovic N (1988) Biotechniques 6: 68–70
Thomas PM (1984) Weeds, insects and diseases. In: Canola Growers Manual. Canola Council of Canada, Winnipeg, Manitoba, Canada, pp. 1045–1048
Venn L (1979) Australas Plant Pathol 8: 5–6
Author information
Authors and Affiliations
Additional information
Communicated by H. Bertrand
Rights and permissions
About this article
Cite this article
Taylor, J.L., Borgmann, I. & Séguin-Swartz, G. Electrophoretic karyotyping of Leptosphaeria maculans differentiates highly virulent from weakly virulent isolates. Curr Genet 19, 273–277 (1991). https://doi.org/10.1007/BF00355054
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00355054