Summary
The characterization of pea chloroplast ribosomal proteins has been carried out. The 30 S and 50 S subunits contain 24 and 32 ribosomal proteins respectively. These numbers are very similar to the numbers previously found for spinach chloroplast (Mache et al. 1980).
Chloroplast-synthesized ribosomal proteins have been determined using the light driven system of protein synthesis of Ellis and Hartley (1981). It is concluded that at least 6 ribosomal proteins of the 30 S and 5 proteins of the 50 S subunits are made in chloroplasts. The possibility that 4 additional ribosomal proteins (3 of the 30 S and 1 of the 50 S subunit) are chloroplastmade, is discussed.
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References
Attardi G (1981) Organization and expression of the mammalian mitochondrial genome: a lesson of economy. Trends in Biochem Sciences 6:86–89
Boynton JE, Gilham NW, Lambovitz AM (1980) Biogenesis of chloroplast and mitochondrial ribosomes. In: Chambliss G, Craven GR, Davies J, Davis K, Kahan L, Nomura M (eds) Ribosomes, structure, function, and genetics. University Park Press, Baltimore, pp 903–950
Chamberlain JP (1979) Fluorographic detection of radioactivity in Polyacrylamide gels with the water-soluble fluor, sodium salicylate. Anal Biochem 98:132–135
Davidson JN, Hanson MR, Bogorad L (1974) An altered chloroplast ribosomal protein in ery-M1 mutants of Chlamydomonas reinhardi. Mol Gen Genet 132:119–129
Ellis RJ, Hartley MR (1982) Preparation of higher plant chloroplasts active in protein and RNA synthesis. In: Edelman M, Hallick RB, Chua NH (eds) Methods in chloroplast molecular biology. Elsevier/North-Holland, Amsterdam (in press)
Freyssinet G (1977) Characterization of cytoplasmic and chloroplast ribosomal proteins of Euglena gracilis. Biochim 59:597–610
Freyssinet G (1978) Determination of the site of synthesis of some Euglena cytoplasmic and chloroplast ribosomal proteins. Exp Cell Res 115:207–219
Gilham NW (1978) Organelle heredity. Raven Press, New York
Hartley MR, Ellis RJ (1973) Ribonucleic acid synthesis in chloroplasts Biochem J 134:249–262
Hartley MR, Head C (1979) The synthesis of chloroplast high-molecular weight ribosomal ribonucleic acid in spinach. Eur J Biochem 96:301–309
Hendler RW (1964) Procedure for simultaneous assay of two β-emitting isotopes with the liquid scintillation counting technique. Anal Biochem 7:110–120
Kaempfer ROR, Meselson M, Raskas HJ (1968) Cyclic dissociation into stable subunits and re-formation of ribosomes during bacterial growth. J Mol Biol 31:277–289
Mache R, Dorne AM, Marti Batlle R (1980) Characterization of spinach plastid ribosomal proteins by two dimensional gel electrophoresis. Mol Gen Genet 177:333–338
Madjar JJ, Michel S, Cozzone AJ, Reboud JP (1979) A method to identify individual proteins in four different two dimensional gel electrophoresis systems. Application to Escherichia coli ribosomal proteins. Anal Biochem 92:174–182
Nomura M, Morgan EA, Jaskunas SR (1977) Genetics of bacterial ribosomes. Annu Rev Genet 11:297–347
Pace NR (1973) Structure and synthesis of the ribosomal ribonucleic acid of procaryotes. Bact Rev 37:562–603
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Communicated by A. Böck
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Eneas-Filho, J., Hartley, M.R. & Mache, R. Pea chloroplast ribosomal proteins: Characterization and site of synthesis. Mol Gen Genet 184, 484–488 (1981). https://doi.org/10.1007/BF00352527
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DOI: https://doi.org/10.1007/BF00352527