Summary
A plasmid-like DNA molecule was isolated from the fungus Ascobolus immersus in strains which exhibited genetic instability. It was found to be inherited maternally. The molecule had a density of 1.708 g/cc and a molecular weight of 4.1×106 daltons. Digestion simultaneously by two restriction enzymes demonstrated the linearity of the molecule. Electron microscopy of alkaline-denatured and self-renatured molecules revealed that the ends are inverted repeated sequences, 1200 base pairs in length.
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Communicated by W. Gajewski
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Francou, F. Isolation and characterization of a linear DNA molecule in the fungus Ascobolus immersus . Mol Gen Genet 184, 440–444 (1981). https://doi.org/10.1007/BF00352519
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DOI: https://doi.org/10.1007/BF00352519