Abstract
We have previously shown that increased sensitivity to diethofencarb in the carbendazim(MBC)-resistant F914 strain of Neurospora crassa is caused by a single amino-acid change in β-tubulin, 198Glu to Gly. Three diethofencarb-resistant mutants that are also resistant to MBC were isolated from strain F914. They contained single base-pair-substitution mutations in the β-tubulin gene. The amino acid changes in β-tubulin, Phe from 250Leu, Val from 165Ala, and Ala from 237Thr, were responsible for diethofencarb-resistance in the mutant strains FR511, FR513, and FR421, respectively. The amino acid at position 198 of β-tubulin in these mutants was Gly, which is the same as in strain F914. β-tubulin genes with 198Glu were constructed by site-directed mutagenesis. The altered β-tubulin genes derived from FR511 and FR421 transformed the wild-type strain to resistance to MBC, indicating that 250Phe and 237Ala in β-tubulin are responsible for resistance not only to diethofencarb but also to MBC.
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Communicated by M. Yamamoto
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Fujimura, M., Kamakura, T., Inoue, H. et al. Amino-acid alterations in the β-tubilin gene of Neurospora crassa that confer resistance to carbendazim and diethofencarb. Curr Genet 25, 418–422 (1994). https://doi.org/10.1007/BF00351780
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DOI: https://doi.org/10.1007/BF00351780