Summary
The addition of 6-azauracil to the growth medium causes a strong reduction of the GTP level in the nucleotide pool of Saccharomyces cerevisiae. In-vitro experiments show a strong inhibition of IMP dehydrogenase activity by 6-azaUMP explaining the preceeding effect. PPR2 mutants, previously characterized by an increased sensitivity to 6-azauracil compared to the wildtype, are specifically susceptible to the lowering of the GTP pool, and are able to grow in presence of 6-azauracil when guanine is added to the medium.
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Communicated by A. Goffeau
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Exinger, F., Lacroute, F. 6-Azauracil inhibition of GTP biosynthesis in Saccharomyces cerevisiae . Curr Genet 22, 9–11 (1992). https://doi.org/10.1007/BF00351735
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DOI: https://doi.org/10.1007/BF00351735