Summary
In one particular myxamoebal strain (NG7; mF+) of Physarum polycephalum, a linear mitochondrial plasmid (mF plasmid) which promotes mitochondrial fusion has been identified. A mating between mF- strains, that do not carry the mF plasmid, resulted in uniparental inheritance of the mtDNA. In matings between mF+ and mF- strains a recombination occurred between the mtDNA and the mF plasmid, and recombinant mtDNA was generated with the end of the mF plasmid as its ends. The DNA sequences of the recombination site in the mtDNA and the mF plasmid, and of the recombinant mtDNA, revealed that the mF plasmid had a 473-bp sequence that was identical to, but slightly shorter than, a 477-bp sequence of the mtDNA. This so-called identical sequence was found at the junction between unique sequences of the mF plasmid and the mtDNA in the recombinant mtDNA. Thus, the recombination between the mtDNA and the mF plasmid was due to reciprocal crossing-over at the identical sequence.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bertrand H, Chan BS-S, Griffiths AJF (1985) Cell 41: 877–884
Bertrand H, Griffiths AJF, Court DA, Cheng CK (1986) Cell 47:829–837
Court DA, Griffiths AJF, Kraus SR, Russell PJ, Bertrand H (1991) Curr Genet 19: 129–137
Dasgupta J, Chan BS-S, Keith MA, Bertrand H (1988) Genome 30 [Suppl 1]: 318
Houchins JP, Ginsburg H, Rohrbaugh M, Dale RMK, Schardl CL, Hodge TP, Lonsdale DM (1986) EMBO J 5: 2781–2788
Kawano S (1991) Bot Mag Tokyo 104:97–113
Kawano S, Kuroiwa T (1989) J Gen Microbiol 135: 1559–1566
Kawano S, Anderson RW, Nanba T, Kuroiwa T (1987) J Gen Microbiol 133: 3175–3182
Kawano S, Takano H, Mori K, Kuroiwa T (1991) Protoplasma 160: 167–169
Lonsdale DM (1989) Biochem Plants 15: 229–295
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Meland S, Johansen S, Johansen T, Haugli K, Haugli F (1991) Curr Genet 19: 55–60
Meinhardt F, Kempken F, Kämper J, Esser K (1990) Curr Genet 17: 89–95
Meyer RJ, Hintz WA, Mohan M, Robinson M, Anderson JB, Horgen PA (1988) Genome 30: 710–716
Myers JC, Griffiths AJF, Bertrand H (1989) Mol Gen Genet 220:113–120
Samac DA, Leong SA (1989) Mol Plant-Microbe Interact 2:155–159
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Schardl CL, Lonsdale SM, Pring DR, Rose KR (1984) Nature 310:292–296
Schardl CL, Pring DR, Lonsdale DM (1985) Cell 43:361–368
Sederoff RR (1984) Adv Genet 22:1–108
Takano H, Kawano S, Suyama Y, Kuroiwa T (1990) Curr Genet 18:125–131
Takano H, Kawano S, Kuroiwa T (1991) Curr Genet 20:315–317
Tudzynski P, Esser K (1986) Curr Genet 10:463–467
Turner G, Earl AJ, Greaves DR (1982) Interspecies variation and recombination of mitochondrial DNA in the Aspergillus nidulans species group and the selection of species-specific sequences by nuclear background. In: Slonimski P, Borst P, Attardi G (eds) Mitochondrial genes. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 411–414
Weissinger AK, Timothy DH, Levings CS III, Hu WWL, Goodman MM (1982) Proc Natl Acad Sci USA 79:1–5
Wolf K, Del Giudice L (1988) Adv Genet 25:185–308
Youngman PJ, Anderson RW, Holt CE (1981) Genetics 97:513–530
Author information
Authors and Affiliations
Additional information
Communicated by K. Esser
Rights and permissions
About this article
Cite this article
Takano, H., Kawano, S. & Kuroiwa, T. Constitutive homologous recombination between mitochondrial DNA and a linear mitochondrial plasmid in Physarum polycephalum . Curr Genet 22, 221–227 (1992). https://doi.org/10.1007/BF00351729
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00351729