Abstract
The exon d of nad1 is located 993 bp upstream of the nad6 gene in the wheat mitochondrial genome. Transcription analyses of both sequences (nad1 exon d and the nad6 gene) were done by Northern hybridization using RNA from wheat seedlings and tissue cultures derived from immature embryos. A complicated pattern was generated with a probe including exon d of nad1 and the whole nad6 gene. An 0.71-kb transcript is specific to nad1 exon d whereas a 1.2-kb transcript is specific to the nad6 gene. Three larger transcripts hybridize to both probes suggesting that nad1 exon d and nad6 are co-transcribed. This co-transcription has been directly demonstrated by cDNA synthesis on mtRNAs and sequencing of the PCR amplification product.
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References
Bland MM, Levings III CS, Matzinger DF (1986) Mol Gen Genet 204:8–16
Bonen L (1993) FASEB J 7:40–46
Chapdelaine Y, Bonen L (1991) Cell 65:465–472
Covello PS, Gray MW (1991) Curr Genet 20:245–251
De Buyser J, Henry Y, Hartmann C, Rode A (1988) Can J Bot 66:1891–1895
Finnegan PM, Brown GG (1990) Plant Cell 2:71–83
Gualberto JM, Wintz H, Weil JH, Grieneberger JM (1988) Mol Gen Genet 215:118–127
Gualberto JM, Bonnard G, Lamattina L, Grieneberger JM (1991) Plant Cell 3:1109–1120
Goldschmidt-Clermont M, Choquet Y, Girard-Bascou J, Michel F, Shirmer-Rahire M, Rochaix JD (1991) Cell 65:135–143
Haouazine N, Pereina de Souza A, Jubier MF, Lancellin D, Delcher E, Lejeune B (1992) Plant Mol Biol 20:395–404
Hartmann C, de Buyser J, Henry, Falconet D, Lejeune B, Benslimane A, Quetier F, Rode A (1987) Plant Sci 53:191–198
Michel F, Dujon B (1983) EMBO J 2:33–38
Michel F, Umesono K, Ozeki H (1989) Gene 82:5–30
Mulligan RM, Leon P, Walbot V (1991) Mol Cell Biol 11:533–543
Pereira de Souza AP, Jubier MF, Delcher E, Lancellin D, Lejeune B (1991) Plant Cell 3:1363–1378
Quetier F, Lejeune B, Delorme S, Falconet D, Jubier MF (1985) In: van Vloten-Doting I, Groot GSP, Hall TC (eds) Molecular form and function of the plant genome. Plenum Press, New York, pp 413–420
Rode A, Hartmann C, Falconet D, Lejeune B, Quetier F, Benslimane A, Henry Y, de Buyser J (1987) Curr Genet 12:369–376
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Stern DB, Newton KJ (1984) Plant Mol Biol Rep 2:8–15
Vedel F, Quetier F (1978) Physiol Vég 16:411–425
Wahleithner JA, MacFarlane JL, Wolstenholme DR (1990) Proc Natl Acad Sci USA 87:548–552
Wissinger B, Hiesel R, Schuster W, Brennicke A (1988) Mol Gen Genet 212:56–65
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Communicated by H. Kössel
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Haouazine, N., Takvorian, A., Jubier, MF. et al. The nad6 gene and the exon d of nad1 are co-transcribed in wheat mitochondria. Curr Genet 24, 533–538 (1993). https://doi.org/10.1007/BF00351718
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DOI: https://doi.org/10.1007/BF00351718