Summary
We have developed a tightly repressed Schizosaccharomyces pombe promoter which can be efficiently induced by Tetracycline. This promoter is a derivative of the plant viral cauliflower mosaic virus 35S promoter which normally functions as a strong constitutive promoter in S. pombe. Location of three binding sites for the Tn10-encoded Tet repressor in the vicinity of the TATA-box of the CaMV 35S promoter led to a tight repression of promoter activity in the presence of the Tet repressor protein. Up to a 400-fold induction was observed after addition of the inducer Tetracycline, which inactivates the operator-binding capacity of the repressor.
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References
Alton KN, Vapnek D (1979) Nature 282:864–869
An G (1987) Methods Enzymol 153:292–305
Bradford MM (1979) Anal Biochem 72:248–254
Beach D, Nurse P (1981) Nature 290:140–142
Fried M, Crothers DM (1981) Nucleic Acids Res 9:6505–6525
Frohberg C, Heins L, Gatz C (1991) Proc Natl Acad Sci USA 88, 10470–10474
Gatz C, Kaiser A, Wendenburg R (1991) Mol Gen Genet 227:229–237
Gatz C, Frohberg C, Wendenburg R (1992) The Plant Journal (in press)
Gmünder H, Kohli J (1989) Mol Gen Genet 220:95–101
Hillen W, Schollmeier K, Gatz C (1984) J Mol Biol 172:185–201
Hoffman CS, Winston F (1989) Gene 84:473–479
Ito H, Fukuda Y, Murata K, Kimura A (1983) J Bacteriol 153:163–168
Kleinschmidt C, Tovar KH, Hillen W, Pörschke D (1988) Biochemistry 27:1094–1104
Maundrell (1990) J Biol Chem 265:10857–10864
Moreno S, Klar A, Nurse P (1991) Methods Enzymol 194:795–823
Oehmichen R, Klock G, Altschmied L, Hillen W (1984) EMBO J 3:539–543
Picard D, Schena M, Yamamoto R (1990) Gene 86:257–261
Pobjecky N, Rosenberg GH, Dinter-Gottlieb G, Käufer NF (1990) Mol Gen Genet 220:314–316
Russell PR (1983) Nature 301:167–169
Russell PR, Hall BD (1983) J Biol Chem 258:143–149
Russell PR, Nurse P (1986) Cell 45:145–155
Tommasino M, Maundrell K (1991) Curr Genet 20:63–66
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Vieira J, Messing J (1987) Methods Enzymol 153:3–17
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Communicated by K. Wolf
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Faryar, K., Gatz, C. Construction of a Tetracycline-inducible promoter in Schizosaccharomyces pombe . Curr Genet 21, 345–349 (1992). https://doi.org/10.1007/BF00351693
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DOI: https://doi.org/10.1007/BF00351693