Summary
Total genomic DNA was isolated from three cereal stem rusts, Puccinia graminis f. sp. tritici, f. sp. secalis, f. sp. avenae, and two cereal leaf rusts, P. recondita f. sp. tritici and P. coronata f. sp. avenae, and analyzed for the presence of heterogeneity in the intergenic region of the ribosomal DNA repeat unit. A 1 kb region of the repeat unit between the 26s and the 5s rRNA genes (IGR-1) was amplified by PCR and was found to be heterogeneous within each isolate and variable in size between races and species. The PCR results were confirmed by Southern blot analysis of native DNA. In an isolate of race C36(48), heterogeneity appeared to be due to variable numbers of 0.1 kb subrepeats in IGR-1. Nine wheat stem rust strains representing nine different races produced a unique pattern of heterogeneity while two different isolates of one race were identical, as were five of another. This may provide a rapid method for race identification in wheat stem rust. Heterogeneity and polymorphism in rye stem rust, oat stem rust, wheat leaf rust, and oat crown rust, was less pronounced than in wheat stem rust. In the course of this work, the 5s rRNA gene was located and its position and orientation within the ribosomal repeat unit was established.
Similar content being viewed by others
References
Burdon JJ, Roelfs AP (1985) Phytopathology 75:907–913
Cassidy JR, Moore D, Lu BC, Pukkila PJ (1984) Curr Genet 8:607–613
Free SJ, Rice PW, Metzenberg RL (1979) J Bacteriol 137:1219–1226
Gutell RR, Fox GE (1988) Nucleic Acids Res 16:r175-r269
Hausner G, Eyjolfsdottir GG, Reid J, Klassen GR (1992) Can J Bot (in press)
Kim WK, Howes NK (1987) Can J Bot 65:1785–1791
Kim WK, Klassen GR (1990) Proceedings 4th International Mycological Congress, Regensburg, Germany, p 195
Kim WK, Martens JW, Howes NK (1984) Can J Plant Pathol 6:111–118
Kim WK, McNabb SA, Klassen GR (1988) Can J Bot 66:1098–1100
Kim WK, Mauthe W, Hausner G, Klassen GR (1990) Can J Bot 68:1898–1902
Klassen GR, McNabb SA, Dick MW (1987) J Gen Microbiol 133:2953–2959
Kohn LM, Petsche DM, Bailey SR, Novak LA, Anderson JB (1988) Phytopathology 78:1047–1051
Long EO, Dawid IB (1980) Annu Rev Biochem 49:727–764
Mullis KB, Faloona F, Scharf S, Saiki R, Horn G, Erlich H (1986) Quant Biol 51:263–273
Myers RM, Sheffield VC, Cox DR (1989) In: Erlich HA (ed) PCR technology. Stockton Press, New York, pp 71–88
Pukkila PJ, Cassidy JR (1987) In: Raynor ADM, Brasier CM, Moore D (eds) Evolutionary biology of the fungi. Cambridge University Press, Cambridge, UK, pp 75–82
Roelfs AP (1984) In: Bushnell WR, Roelfs AP (eds) The cereal rusts, vol 1. Academic Press, Orlando, Florida, pp 131–164
Rogers SI, Rehner S, Bledsoe C, Mueller GJ, Ammirati JF (1989) Can J Bot 67:1235–1243
Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1985) Science 239:487–491
Selker EU, Stevens JN, Metzenberg RL (1985) Science 227:1340–1343
Walker WF, Doolittle WF (1982) Nature 299:723–724
Wolters J, Erdmann VA (1988) Nucleic Acids Res 16:r1-r70
Author information
Authors and Affiliations
Additional information
Communicated by O. C. Yoder
Rights and permissions
About this article
Cite this article
Kim, W.K., Zerucha, T. & Klassen, G.R. A region of heterogeneity adjacent to the 5s ribosomal RNA gene of cereal rusts. Curr Genet 22, 101–105 (1992). https://doi.org/10.1007/BF00351468
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00351468