Abstract
Seventeen linking clones sublocalized to the central region of the mouse X Chromosome (Chr) were screened against genomic DNA from male mice carrying the tabby-25H (Ta 25H) deletion. Two of these linking clones, λEM131 and λEM169, were found to be deleted in Ta 25H/Y animals. Genetic mapping through Mus musculus domesticus/Mus spretus interspecific backcross progeny, segregating for the original tabby (Ta) gene mutation, was utilized to order these markers and to define nearest flanking markers to the Ta 25H deletion (λEM140 and λEM171). The size of the Ta 25H deletion was thus estimated as up to 4.5 centiMorgans (cM). The order of markers, proximal to distal, was found to be λEM140/λEM131, mouse androgen receptor gene (Ar)/λEM169, Ta/λEM171. A putative CpG-rich island and a highly evolutionarily conserved DNA probe were isolated from the DXCrc169 locus which co-segregates with the Ta locus in this study.
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Brockdorff, N., Kay, G., Cattanach, B.M. et al. Molecular genetic analysis of the Ta 25H deletion: Evidence for additional deleted loci. Mammalian Genome 1, 152–157 (1991). https://doi.org/10.1007/BF00351061
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DOI: https://doi.org/10.1007/BF00351061