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Intracellular localization of vanadium in the fan worm Pseudopotamilla occelata

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Abstract

A very high level of vanadium was found to be contained in the polychaete worm Pseudopotamilla occelata collected in 1992–1993 from the Sanriku coast on the main island of Japan. The vanadium concentration (mean±SD=5500±1800 μg g-1 dry wt) in the worm's branchial crown which is composed of many bipinnate radioles was approximately 100 times higher than that (mean±SD=60±25 μg g-1 dry wt) in the trunk body. Electron probe X-ray microanalysis revealed that a large amount of vanadium was present in the outer potion of the epidermis of the bipinnate radiole. Analytical electron microscopy for a cryo-section of the bipinnate radioles indicated that vanadium was localized in electron-dense deposits in the apical portion of epidermal cells. From an examination of the fine structures, the locality of the electron-dense deposits were found to correspond to that of the apical vacuoles in the epidermal cells. It was concluded that most of the vanadium in P. occelata was contained in the vacuoles of the epidermal cells of the bipinnate radioles.

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Authors and Affiliations

  1. Marine Radioecology, National Institute of Radiological Sciences, 3609 Isozaki, Nakaminato, 311-12, Japan

    T. Ishii, M. Nakahara & R. Nakamura

  2. Ocean Research Institute, University of Tokyo, Nakano, 164, Tokyo, Japan

    T. Otake

  3. Department of Biotechnology, Senshu University of Ishinomaki, Minamisakai, 986, Ishinomak, Japan

    K. Okoshi

Authors
  1. T. Ishii
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  2. T. Otake
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  3. K. Okoshi
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  4. M. Nakahara
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  5. R. Nakamura
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Communicated by T. Ikeda, Nagasaki

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Ishii, T., Otake, T., Okoshi, K. et al. Intracellular localization of vanadium in the fan worm Pseudopotamilla occelata . Marine Biology 121, 143–151 (1994). https://doi.org/10.1007/BF00349483

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  • DOI: https://doi.org/10.1007/BF00349483

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