Abstract
The major concentrations of human satellite DNA I (1.688 g/ml) have been localised on human chromosome preparations by the technique of in situ hybridisation using radioactive complementary RNA synthesised in vitro. Chromosomes were identified by prior study using quinacrine fluorescence microscopy. The satellite DNA is concentrated, mainly in centromeric constitutive heterochromatin, on many chromosomes but is especially obvious in the fluorescent distal segment of the Y chromosome.
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Birnstiel, M., Spiers, J., Purdom, I., Jones, K.W., Loening, U.E.: Properties and composition of the isolated ribosomal DNA satellite of Xenopus Laevis. Nature (Lond.) 219, 454–463 (1968)
Birnstiel, M. L., Sells, B. H., Purdom, I. F.: Kinetic complexity of RNA molecules. J. molec. Biol. 63, 21–39 (1972)
Bishop, J. O.: The effect of genetic complexity on the time-course of ribonucleic acid — deoxyribonucleic acid hybridisation. Biochem. J. 113, 805–811 (1969)
Corneo, G., Ginelli, E., Polli, E.: Isolation of the complementary strands of a human satellite DNA. J. molec. Biol. 33, 331–335 (1968)
Corneo, G., Ginelli, E., Polli, E.: Repeated sequences in human DNA. J. molec. Biol. 48, 319–327 (1970)
Corneo, G., Ginelli, E., Polli, E.: Renaturation properties and localisation in heterochromatin of human satellite DNAs. Biochim. biophys. Acta (Amst.) 247, 528–534 (1971)
Flamm, W. G., Walker, P. M. B., McCallum, M.: Some properties of the single strands isolated from the DNA of the molecular satellite of the mouse. J. molec. Biol. 40, 423–443 (1969)
Gall, J. G., Pardue, M. L.: Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc. nat. Acad. Sci. (Wash.) 63, 378–383 (1969)
Gillespie, D., Spiegelman, S.: A quantitative assay for DNA-RNA hybrids with DNA immobilised on a membrane. J. molec. Biol. 12, 829–842 (1966)
Henderson, A., Warburton, D., Atwood, K. C.: Localisation of ribosomal DNA in the human chromosome complement. Proc. nat. Acad. Sci. (Wash.) 69, 3394–3398 (1972)
John, H. A., Birnstiel, M., Jones, K. W.: RNA/DNA hybrids at the cytological level. Nature (Lond.) 223, 582–587 (1969)
Jones, K. W.: Chromosomal and nuclear location of mouse satellite DNA in individual cells. Nature (Lond.) 225, 912–915 (1970)
Jones, K. W.: Annotation: Satellite DNA. J. med. Genet. 10, 273–281 (1973)
Jones, K. W.: Primate satellite DNA. In: World anthropology (Sol Tax, ed.). The Hague: Mouton 1974
Jones, K. W., Corneo, G.: Location of satellite and homogeneous DNA sequences on human chromosomes. Nature (Lond.) New Biol. 233, 268–271 (1971)
Jones, K. W., Prosser, J., Corneo, G., Ginelli, E.: The chromosomal location of human satellite DNA III. Chromosoma (Berl.) 42, 445–451 (1973)
Jones, K. W., Prosser, J., Corneo, G., Ginelli, E., Bobrow, M.: Satellite DNA, constitutive heterochromatin and human evolution. In: Modern aspects of cytogenetics: Constitutive heterochromatin in man (R. A. Pfeiffer, ed.), p. 45–61, Stuttgart-Hew York: F. K. Schattauer Verlag 1973)
Jones, K. W., Robertson, F. W.: Localisation of reiterated nucleotide sequences in Drosophila and mouse by in situ hybridisation of complementary RNA. Chromosoma (Berl.) 31, 331–345 (1970)
Latt, S. A., Davidson, R. L., Lin, M. S., Gerald, P. S.: Lateral asymmetry in the fluorescence of human Y chromosomes strained with 33258 Hoechst. Exp. Cell Res. (In press)
Lin, C. C., Uchida, I. A., Byrnes, E.: A suggestion for the nomenclature of the fluorescent banding patterns in human metaphase chromosomes. Canad. J. Genet. Cytol. 13, 361–363 (1971)
Lin, M. S., Latt, S. A., Davidson, R. L.: Identification of human and mouse chromosomes in human-mouse hybrids by centromere fluorescence. Exp. Cell Res. 87, 429–433 (1974)
Marmur, J.: A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. molec. Biol. 3, 208–218 (1961)
Pardue, M. L., Gall, J. G.: Chromosomal localisation of mouse satellite DNA. Science 168, 1356–1368 (1970)
Pearson, P. L., Bobrow, M., Vosa, C. G., Barlow, P. W.: Quinacrine fluorescence in Mammalian chromosomes. Nature (Lond.) 231, 326–329 (1971)
Prosser, J.: Satellite DNA in man and three higher primates. Ph. D. Thesis, Edinburgh University (1974)
Prosser, J., Moar, M., Bobrow, M., Jones, K. W.: Satellite DNA sequences in the chimpanzee (Pan troglodytes). Biochim. biophys. Acta (Amst.) 319, 122–134 (1973)
Turleau, C., Grouchy, J. de: Caryotypes de l'homme et du chimpanze. Comparison de la topographie des bandes. Méchanismes évolutifs possibles. C. R. Acad. Sci. (Paris) 274, 2355–2357 (1972)
Vinograd, J., Morris, J., Davidson, N., Dove, W.: The buoyant density behaviour of viral and bacterial DNA in alkaline CsC1. Proc. nat. Acad. Sci. (Wash.) 49, 12–17 (1963)
Wells, R. D., Blair, J. E.: Studies of polynucleotides. LXXI. Sedimentation and buoyant density studies of some DNA-like polymers with repeating nucleotide sequences. J. molec. Biol. 27, 273–288 (1967)
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Jones, K.W., Purdom, I.F., Prosser, J. et al. The chromosomal localisation of human satellite DNA I. Chromosoma 49, 161–171 (1974). https://doi.org/10.1007/BF00348888
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DOI: https://doi.org/10.1007/BF00348888