Summary
The early neurogenesis of rat neocortex was analysed by means of light and electron microscopic autoradiography. It was found that the very first preneurons originate probably as early as ED 11. They are the horizontal cells of Cajal-Retzius. The peak of their formation is on ED 13 (surface index estimated on ED 17 after injection of 3H-thymidine on ED 13:21, after injection on ED 12:4, after injection on ED 15:5), whereas no Cajal-Retzius cells could be found to have originated after ED 15. These cells are the developmentally most advanced of the neocortex. The cells second in date of origin and maturation are preneurons which presumably correspond to the presumptive neurons of Layer VII (VI b), and begin to originate on ED 12. The end of their formation could not be defined owing to a lack of ultrastructural differences to other, younger preneurons in later gestational stages. These two cell types are the first cellular components of the primordial plexiform layer (Marin-Padilla, 1978) or pallial anlage (Rickmann, 1977), demonstrating an outside-in gradient within this layer, and are separated by the formation of the cortical plate. This could be proven by their simultaneous labelling above and below the cortical plate after administration of 3H-thymidine before ED 15. These results confirm the hypothesis of a dual origin of the mammalian neocortex (Marin-Padilla, 1978).
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References
Angevine, J.B., Sidman, R.L.: Autoradiographic study of histogenesis in the cerebral cortex of the mouse. Anat. Rec. 142, 210 (1962)
Bär, T., Wolff, J.R.: The formation of capillary basement membranes during internal vascularisation of the rat's cerebral cortex. Z. Zellforsch. 133, 231–248 (1972)
Berry, M., Rogers, A.W.: The migration of neuroblasts in the developing cerebral cortex. J. Anat. 99, 691–709 (1965)
Berry, M., Rogers, A.W.: Histogenesis of mammalian neocortex. In: Evolution of the forebrain, (Hassler, R. and Stephan, H., eds.) pp. 197–205. New York: Plenum Press 1967
Bisconte, J.-C., Marty, R.: Analyse chronoarchitectonique du cerveau de rat par radioautographie. I. Histogenèse du télencéphale. J. Hirnforsch. 16, 55–74 (1975)
Boulder Committee: Embryonic vertebrate central nervous system: revised terminology. Anat. Rec. 166, 257–261 (1970)
Bradford, R., Parnavelas, J.G., Liebermann, A.R.: Neurons in Layer I of the developing occipital cortex of the rat. J. Comp. Neur. 176, 121–132 (1977)
Brückner, G., Mareš, V., Biesold, D.: Neurogenesis in the visual system of the rat. An autoradiographic investigation. J. Comp. Neur. 166, 245–256 (1976)
Derer, P., Caviness, V.S., Sidman, R.L.: Early cortical histogenesis in the primary olfactory cortex of the mouse. Brain Res. 123, 27–40 (1977)
Duckett, S., Pearse, A.G.E.: The cells of Cajal-Retzius in the developing human brain. J. Anat. 102, 183–187 (1968)
Fischer, H.A.: Autoradiography of specimens for electron microscopy-presentation of a method with special remarks on the use of samples from the nervous system. Brain Res. 85, 237–240 (1975)
Fujita, S.: Applications of light and electron microscopy, autoradiography to the study of cytogenesis of the forebrain. In: Evolution of the forebrain, (Hassler, R. and Stephan, H., eds.), pp. 180–196. New York: Plenum Press 1967
Hicks, S.P., D'Amato, C.J.: Cell migration to the isocortex in the rat. Anat. Rec. 160, 619–634 (1968)
Kahle, W.: Studien über die Matrixphasen und die örtlichen Reifungsunterschiede im embryonalen menschlichen Gehirn. Dt. Zschr. Nervenheilk. 166, 273–302 (1951)
Kennedy, L.A., Persaud, T.V.N.: Pathogenesis of developmental defects induced in the rat by amniotic sac puncture. Acta Anat. 97, 23–35 (1977)
König, N., Roche, G., Marty, R.: The onset of synaptogenesis in rat temporal cortex. Anat. Embryol. 148, 73–87 (1975)
König, N., Valat, J., Fulcrand, J., Marty, R.: The time of origin of Cajal-Retzius cells in the rat temporal cortex. An autoradiographic study. Neurosc. Lett. 4, 21–26 (1977)
Langman, J., Welch, G.W.: Excess vitamin A and development of the cerebral cortex. J. Comp. Neur. 131, 15–25 (1967)
Luft, J.H.: Improvements in epoxy resin embedding methods. J. Biophys. Biochem. Cytol. 9, 409–414 (1961)
Lund, J.S., Boothe, R.G., Lund, R.D.: Development of neurons in the visual cortex (area 17) of the monkey (macaca nemestrina): A Golgi study from fetal day 127 to postnatal maturity. J. Comp. Neur. 176, 149–188 (1977)
Lyser, K.J.: Early differentiation of motor neuroblasts in the chick embryo as studied by electron microscopy. I. General aspects. Developm. Biol. 10, 433–466 (1964)
Marin-Padilla, M.: Early prenatal ontogenesis of the cerebral cortex (neocortex) of the cat (felis domestica). A Golgi study. I. The primordial neocortical organisation. Z. Anat. Entwickl.-Gesch. 134, 117–145 (1971)
Marin-Padilla, M.: Prenatal ontogenetic history of the principal neurons of the neocortex of the cat (felis domestica). A Golgi study. II. Developmental, differences and their significances. Z. Anat. Entwickl.-Gesch. 136, 125–142 (1972)
Marin-Padilla, M.: Dual origin of the mammalian neocortex and evolution of the cortical plate. Anat. Embryol. 152, 109–126 (1978)
Meller, K., Tetzlaff, W.: Neuronal migration during the early development of the cerebral cortex. A scanning electron microscopic study. Cell Tiss. Res. 163, 313–325 (1975)
Morest, D.K.: The growth of dendrites in the mammalian brain. Z. Anat. Entwickl.-Gesch. 128, 290–317 (1969)
Morest, D.K.: A study of neurogenesis in the forebrain of opossum pouch young. Z. Anat. Entwickl.-Gesch. 131, 45–67 (1970)
Raedler, A., Sievers, J.: The development of the visual system of the albino rat. Adv. Anat. Embryol. Cell Biol. 50, Fasc. 3 (1975)
Raedler, A., Sievers, J.: Light and electron microscopical studies on specific cells of the marginal zone in the developing rat cerebral cortex. Anat. Embryol. 149, 173–181 (1976)
Rakic, P.: Genesis of the dorsal lateral geniculate nucleus in the Rhesus monkey: Site and time of origin, kinetics of proliferation, routes of migration and pattern of distribution of neurons. J. Comp. Neur. 176, 23–52 (1977)
Rickmann, M., Chronwall, B.M., Wolff, J.R.: On the development of nonpyramidal neurons and axons outside the cortical plate: The early marginal zone as a pallial anlage. Anat. Embryol. 151, 285–307 (1977)
Schultze, B., Maurer, W., Hagenbusch, H.: A two emulsion autoradiographic technique and the discrimination of the three different types of labelling after double labelling with 3H-and 14C-thymidine. Cell Tissue Kinet. 9, 245–255 (1976)
Shimada, M., Langman, J.: Cell proliferation, migration and differentiation in the cerebral cortex of the golden hamster. J. Comp. Neur. 139, 227–244 (1970)
Sidman, R.L., Miale, I.L., Feder, N.: Cell proliferation and migration in the primitive ependymal zone: An autoradiographic study of histogenesis in the nervous system. Exper. Neurol. 1, 322–333 (1959)
Smart, I.H.M.: Proliferative characteristics of the ependymal layer during the early development of the mouse neocortex: a pilot study based on recording the number, location and plane of cleavage of mitotic figures. J. Anat. 116, 67–91 (1973)
Smart, J.H.M., Smart, M.: The location of nuclei of different labelling intensity in autoradiographs of the anterior forebrain of postnatal mice injected with 3H-thymidine on the eleventh and twelfth days post-conception. J. Anat. 123, 515–525 (1977)
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Raedler, E., Raedler, A. Autoradiographic study of early neurogenesis in rat neocortex. Anat. Embryol. 154, 267–284 (1978). https://doi.org/10.1007/BF00345657
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DOI: https://doi.org/10.1007/BF00345657