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Postoperative biosynthetic human growth hormone increases the strength and collagen deposition of experimental colonic anastomoses

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Abstract

This study examined the effects of preoperative treatment with 2.0 mg biosynthetic human growth hormone (b-hGH)/kg/day on the bursting strength and collagen deposition of experimental left colonic anastomoses, and of intact colon from sham-operated rats. The anastomtic bursting pressure was 55% higher in the b-hGH treated animals on day 2 (p<0.05) and 79% higher on day 4 (NS; p=0.056), and the bursting wall tension was 65% higher on day 2 (p<0.05) and 112% higher on day 4 postoperatively (p<0.05), than saline-injected controls. The hydroxyproline content of the anastomotic segment in the b-hGH treated rats increased by 56% on day 4 (p<0.005) and by 30% on day 6 postoperatively (p<0.05), compared with controls. At 3 cm proximal to the anastomoses the defatted dry weight and hydroxyproline content of the healing colons were increased after 6 days compared with the sham-operated rats. There was, however, no difference between the b-hGH treated rats and the paired controls, indicating that growth hormone is not involved in this process.

Résumé

Ce travail étudie les effects d'un traitement avec 2 mg d'hormone de croissance humaine bio-synthétique (b-hGH)/kg/jour commencé avant l'intervention sur la résistance à la rupture et le dépot de collagène des anastomoses coliques gauches expérimentales et du colon intact chez des rats opérés fictivement. La pression de rupture anastomotique était 55% plus élevée chez les animaux traités par b-hGH au deuxième jour (p<0.05) et 79% plus élevée au jour 4 (NS, p=0.056), et la tension de rupture pariétale était 65% plus élevée au jour 2 (p<0.05) et 112% plus élevée au quatrième jour post-opératoire (p<0.05) que chez les contrôles. Le contenu en hydroxyproline du segment anastomotique chez les animaux traités augmentait de 56% au 4ème jour (p<0.005) et de 30% au sixième jour post-opératoire (p<0.05), comparé avec les contrôles. A trois centimètres en amont des anastomoses les faits démontraient que le poid sec dégressé et le contenu en hydroxyproline des colons cicatrisés était accru après le sixi⪻me jour en comparaison avec les rats opérés fictivement. Il n'y avait cependant aucune diffécence entre les rats traités et les contrôles appareillés indiquant que l'hormone de croissance n'est pas intéressée par ce processus.

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Authors and Affiliations

  1. Department of Connective Tisue Biology, Institute of Anatomy, University of Aarhus, Aarhus, Denmark

    Henrik Christensen & Hans Oxlund

  2. Institute of Experimental Clinical Research, University of Aarhus, Aarhus, Denmark

    Henrik Christensen & Søren Laurberg

Authors
  1. Henrik Christensen
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  2. Hans Oxlund
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  3. Søren Laurberg
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Christensen, H., Oxlund, H. & Laurberg, S. Postoperative biosynthetic human growth hormone increases the strength and collagen deposition of experimental colonic anastomoses. Int J Colorect Dis 6, 133–138 (1991). https://doi.org/10.1007/BF00341232

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