Summary
Thirteen male human foetuses ranging in crown-rump length from 29 to 212 mm (ages 8–27 weeks) were studied. Four developmental phases are distinguished. 1. The predifferentiation phase (below 8 weeks): The interstitium contains only undifferentiated mesenchymal cells. 2. The differentiation phase (8–14 weeks): Leydig cells develop and gradually fill the space between the germ cords. 3. The maturity phase (14–18 weeks): The interstitium occupies more than one half of the total area in the testis sections and is filled with mature foetal Leydig cells. 4. The involution phase (18–40 weeks): Most of the Leydig cells gradually degenerate and disappear.
The foetal Leydig cells are packed with tubular agranular endoplasmic reticulum (AER). Islets of parallel granular ER membranes and other organelles are embedded in the AER. The mitochondria vary in shape and form, the cristae being mainly tubular. Some mitochondria like organelles contain electron dense inclusions. Dark membrane bound bodies of variable form and resembling the Golgi cisternae are present in most cells. Reinke crystals are never found in the foetal cells. In degenerating Leydig cells the AER appears in vesicular form, membranous whorls are seen in some of them and the cell membrane seems to rupture finally, and cytoplasmic material protrudes outside the cells. The fine structure of the mature foetal Leydig cells is suggested to reflect signs of human chorionic gonadotrophin stimulation.
Similar content being viewed by others
References
Acevedo, H. F., L. R. Axelrod, E. Ishikava, and F. Takaki: Studies in fetal metabolism. II. Metabolism of progesterone-4-C14 and pregnenolone-7α-H3 in human fetal testes. J. clin. Endocr. 23, 885–890 (1963).
Baillie, A. H., M. M. Ferguson, and D. McK. Hart: The genital ridge and indifferent gonad. In: Developments in steroid histochemistry, p. 34–38. London: Academic Press 1966.
Bennett, H. S., and J. H. Luft: S-collidine as a basis for buffering fixatives. J. biophys. biochem. Cytol. 6, 113–114 (1959).
Bjersing, L.: On the ultrastructure of granulosa lutein cells in porcine corpus luteum. Z. Zellforsch. 82, 187–211 (1967).
Black, V. H.: Differentiation of interstitial cells in fetal guinea pig testes. Anat. Rec. 157, 214 (1967).
Brökelmann, J.: Über die Stütz- und Zwischenzellen des Froschhodens während des spermatogenetischen Zyklus. Z. Zellforsch. 64, 429–461 (1964).
Carr, I., and J. Carr: Membranous whorls in the testicular interstitial cell. Anat. Rec. 144, 143–147 (1962).
Christensen, A. K.: The fine structure of testicular interstitial cells in guinea pigs. J. Cell Biol. 26, 911–935 (1965).
Fawcett, D. W., and M. H. Burgos: Studies on the fine structure of the mammalian testis. II. The human interstitial tissue. Amer. J. Anat. 107, 245–269 (1960).
Fawcett, D. W., and M. H. Burgos: Observation on the cytomorphosis of the germinal and interstitial cells of the human testis. Ciba Foundation Colloquia on Ageing, vol. 2, p. 86–99. London: J. & A. Churchill 1956.
Gillman, J.: The development of the gonads in man, with a consideration of the role of the fetal endocrines and the histogenesis of ovarian tumors. Contr. Embryol. Carneg. Instn 32, 81–131 (1948).
Gordon, G. B., L. R. Miller, and K. G. Bensch: Electron microscopic observations of the gonad in the testicular feminization syndrome. Lab. Invest. 13, 152–160 (1964).
Hamilton, W. J., J. D. Boyd, and H. W. Mossman: Human embryology, 3rd. ed., p. 120. Cambridge: W. Heffer & Sons Ltd. 1962.
Hatakeyama, S.: A study on the interstitial cells of the human testis, especially on their fine-structural pathology. Acta path. jap. 15, 155–197 (1965).
Ikonen, M.: On the structure and function of human testicular interstitial cells in the embryonal stage. A thesis. Helsinki, Finland (1965).
—, and M. Niemi: Metabolism of progesterone and 17α-hydroxypregnenolone by the foetal human testis in vitro. Nature (Lond.) 212, 716–717 (1966).
Jost, A.: Gonadal hormones in the sex differentiation of the mammalian fetus. In: Organogenesis, chap. 24, p. 611–628. New York: Holt, Rinehart & Winston 1965.
Kretser, D. M. de: The fine structure of the testicular interstitial cells in men of normal androgenic status. Z. Zellforsch. 80, 594–609 (1967a).
—: Changes in the fine structure of the human testicular interstitial cells after treatment with human gonadotrophins. Z. Zellforsch. 83, 344–358 (1967b).
Leydig, F.: Zur Anatomie der männlichen Geschlechtsorgane und Analdrüsen der Säugetiere. Z. wiss. Zool. 2, 1–57 (1850).
Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961).
Merkow, L. H., F. Acevedo, M. Slifkin, and B. J. Caito: Studies on the interstitial cells of the testis. I. The ultrastructure in the immature guinea pig and the effect of stimulation with HCG. Amer. J. Path. 53, 47–61 (1968a).
—, and M. Pard: Studies on the interstitial cells of the testis. II. The ultrastructure in the adult guinea pig and the effect of stimulation with human chorionic gonadotrophin. Amer. J. Path. 53, 989–1007 (1968b).
Narbaitz, R., and R. Adler: Submicroscopical aspects in the differentiation of rat fetal Leydig cell. Acta physiol. lat.-amer. 17, 286–291 (1967).
Niemi, M., M. Ikonen, and A. Hervonen: Histochemistry and fine structure of the interstitial tissue in the human foetal testis. Ciba Foundation Colloquia on Endocrinology, Endocrinology of the testis, vol. 16, p. 31–55. London: J. & A. Churchill Ltd. 1967.
Ottowicz, J.: The stadial development of Leydig cells. Acta med. pol. 1, 1–14 (1963).
Reinke, Fr.: Über Kristalloidbildungen in den interstitiellen Zellen des menschlichen Hodens. Arch. mikr. Anat. 47 (1896).
Smith, R. E., and M. G. Farquhar: Lysosome function in regulation of the secretory process in cells of the anterior pituitary gland. J. Cell Biol. 31, 319–347 (1966).
Sniffen, R. C.: The testis. I. The normal testis. Arch. Path. 50, 259–284 (1950).
—, R. P. Howard, and F. A. Simmons: The testis. II. Abnormalities of spermatogenesis; Atresia of excretory ducts. Arch. Path. 50, 285–295 (1950).
Venable, J. H., and R. Coggeshall: A simplified lead citrate stain for use in electron microscopy. J. Cell Biol. 25, 407–408 (1965).
Wagenen, G. van, and M. E. Simpson: Embryology of the ovary and testis. Homo sapiens and Macaca, mulatta. New Haven, USA: Yale University Press 1965.
Watson, M. L.: Staining of tissue sections for electron microscopy with heavy metals. J. biophys. biochem. Cytol. 4, 475–478 (1958).
Wide, L.: An immunological method for the assay of human chorionic gonadotrophin. Acta endocr. (Kbh.), Suppl. 70, 81 (1962).
Winiwarter, H. von: Observations cytologiques sur les cellules interstitielles du testicule humain. Anat. Anz. 41, 309–320 (1912).
Yamada, E.: Some observations on the fine structure of the interstitial cells in the human testis as revealed by electron microscopy. Gunma Symposium on Endocrinology, vol. 2, p. 1 Maebashi, Japan: Gunma University Institute of Endocrinology 1965.
—: Some observations on the fine structure of the interstitial cell in the human testis. Fifth Internat. Congr. for Electron Microscopy, Philadelphia, vol. 2, p. LL 1. New York: Academic Press 1962.
Yasuzumi, G., Y. Nakai, I. Tsubo, M. Yasuda, and T. Sugioka: The fine structure of nuclei as revealed by electron microscopy. IV. The intranuclear inclusion formation in Leydig cells of ageing human testes. Exp. Cell Res. 45, 261–276 (1967).
Zondek, L. H., and T. Zondek: Leydig cells of the fetus and newborn in normal and toxaemic pregnancy. Biol. Neonat. 8, 1–22 (1965).
Author information
Authors and Affiliations
Additional information
This investigation was supported by the Damon Runyon Memorial Fund (DRG-940) and by the Sigrid Jusélius Foundation.
Rights and permissions
About this article
Cite this article
Pelliniemi, L.J., Niemi, M. Fine structure of the human foetal testis. Z. Zellforsch. 99, 507–522 (1969). https://doi.org/10.1007/BF00340942
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF00340942