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Separation of chromosomal DNA molecules from Phoma tracheiphila by orthogonal-field-alternation gel electrophoresis

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Summary

Chromosomal DNA molecules from the plant pathogenic fungus Phoma tracheiphila were separated by orthogonal-field-alternation gel electrophoresis (OFAGE). Intact chromosomal DNA was obtained by a protocol which involves embedding fungal hyphae in low-gelling agarose followed by enzymatic digestion of the cell wall and subsequent lysis of the other cell components by proteinase k and sarcosyl. The electrophoretic karyotype revealed the presence of twelve distinct bands.

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Authors and Affiliations

  1. Dipartimento di Biologia Cellulare, via F. Camerini 2, I-62032, Camerino, Italy

    Franco Rollo, Tina Ferracuti & Aurelio Pacilli

Authors
  1. Franco Rollo
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  2. Tina Ferracuti
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  3. Aurelio Pacilli
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Rollo, F., Ferracuti, T. & Pacilli, A. Separation of chromosomal DNA molecules from Phoma tracheiphila by orthogonal-field-alternation gel electrophoresis. Curr Genet 16, 477–479 (1989). https://doi.org/10.1007/BF00340730

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  • DOI: https://doi.org/10.1007/BF00340730

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