Summary
The product of the PHO85 gene, which encodes one of the negative regulatory factors of the PHO system in Saccharomyces cerevisiae, shows significant amino acid sequence homology with the CDC28 protein kinase. However, overexpressing PHO85 did not suppress the temperature sensitive phenotype of the cdc28-1 mutation. The nucleotide sequence of the PHO85 gene strongly suggests the presence of an intron near the sequence encoding the N-terminal region.
References
Celenza JL, Carlson M (1986) A yeast gene that is essential for release from glucose repression encodes a protein kinase. Science 233:1175–1180
Gallwitz D, Sures I (1980) Structure of a split yeast gene: complete nucleotide sequence of the actin gene in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 77:2546–2550
Hindley J, Phear GA (1984) Sequence of the cell division gene CDC2 from Schizosaccharomyces pombe; patterns of splicing and homology to protein kinases. Gene 31:129–134
Hunter T, Cooper JA (1985) Protein-tyrosine kinases. Annu Rev Biochem 54:897–930
Legrain M, De-Wilde M, Hilger F (1986) Isolation, physical characterization and expression analysis of the Saccharomyces cerevisiae positive regulatory gene PHO4. Nucleic Acids Res 14:3059–3073
Levin DE, Hammond CI, Ralston RO, Bishop JM (1987) Two yeast genes that encode unusual protein kinases. Proc Natl Acad Sci USA 84:6035–6039
Lörincz AT, Reed SI (1984) Primary structure homology between the product of yeast cell division control gene CDC28 and vertebrate oncogenes. Nature 307:183–185
Matsumoto K, Uno I, Ishikawa T (1984) Regulation of repressible acid phosphatase by cyclic AMP in Saccharomyces cerevisiae. Genetics 108:53–66
Matsumoto K, Uno I, Ishikawa T (1985) Genetic analysis of the role of cAMP in yeast. Yeast 1:15–24
Miller AM (1984) The yeast MATa1 gene contains two introns. EMBO J 3:1061–1065
Ng R, Abelson J (1980) Isolation and sequence of the gene for actin in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 77:3912–3916
Simon M, Seraphin B, Faye G (1986) KIN28, a yeast split gene coding for a putative protein kinase homologous to CDC28. EMBO J 5:2697–2701
Sternberg MJE, Taylor WR (1984) Modeling the ATP-binding site of oncogene products, the epidermal growth factor receptor and related proteins. FEBS Lett 175:387–392
Tanaka K, Matsumoto K, Toh-e A (1988) Dual regulation of the expression of the polyubiquitin gene by cyclic AMP and heat shock in yeast. EMBO J 7:495–502
Teague MA, Chaleff DT, Errede B (1986) Nucleotide sequence of the yeast regulatory gene STE7 predicts a protein homologous to protein kinases. Proc Natl Acad Sci USA 83:7371–7375
Teem J, Abovich N, Kaufer N, Schwindinger W, Warner J, Levy A, Woolford J, Leer R, van Raamsdonk-Duin M, Mager W, Planta R, Schultz L, Friesen J, Fried H, Rosbash M (1984) A comparison of yeast ribosomal protein gene DNA sequences. Nucleic Acids Res 12:8295–8312
Toh-e A, Shimauchi T (1986) Cloning and sequencing of the PHO80 gene and CEN15 of Saccharomyces cerevisiae. Yeast 2:129–139
Uesono Y, Tanaka K, Toh-e A (1987) Negative regulators of the PHO system in Saccharomyces cerevisiae: isolation and structural characterization of PHO85. Nucleic Acids Res 15:10299–10309
Author information
Authors and Affiliations
Additional information
Communicated by C.P. Hollengerg
Rights and permissions
About this article
Cite this article
Toh-e, A., Tanaka, K., Uesono, Y. et al. PHO85, a negative regulator of the PHO system, is a homolog of the protein kinase gene, CDC28, of Saccharomyces cerevisiae . Mol Gen Genet 214, 162–164 (1988). https://doi.org/10.1007/BF00340196
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00340196