Summary
The incidence of ring shaped nucleoli was studied by means of light and electron microscopical methods in liver cells of rats injected intravenously with Actinomycin D in a dose of 150 μg per kg body weight.
Ring shaped nucleoli were most frequent 20 minutes after administration of Actinomycin D. The number of fragmented nucleoli was increased 20 minutes after administration of the drug and did not decrease even after 72 hours.
Fibrillar ring like inclusions within nuclei and more perichromatin granules at the periphery of nucleoli were observed after treatment with Actinomycin D.
Zusammenfassung
Die Wirkung von Aktinomycin D (150 μg/kg) auf die Leberzellen der Ratte wurde licht- und elektronenenmikroskopisch untersucht. 20 min nach intravenöser Aktinomycin-D-Gabe wurden ringörmige Nukleolen beobachtet. Die Zahl der fragmentierten Nukleolen war 20 min nach der Applikation gesteigert. Ihre relative Zahl änderte sich nach Aktinomycingabe nicht. Nach Behandlung der Tiere mit Aktinomycin D kann man in den Kernen der Leberzellen fibrilläre ringförmige Einschlüsse und eine größere Menge von „perichromatin-granules“ an der Peripherie der Nukleolen beobachten.
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The authors are indebted to M. Mikolášová, V. Povolná, I. Černíková and Dr. M. Titlbach for their help, to Dr. J. Chaloupka for providing Actinomycin D and to Professor Busch and Professor Wolf for many helpful suggestions.
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Smetana, K., Potměšil, M. Ring shaped nucleoli in liver cells of rats after treatment with Actinomycin D. Zeitschrift für Zellforschung 92, 62–69 (1968). https://doi.org/10.1007/BF00339403
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DOI: https://doi.org/10.1007/BF00339403