Summary
The synthesis of mucopolysaccharides in the gland of Brunner was investigated through the use of tritium labelled glucose by electronmicroscopic-autoradiography. Mice of 20 gm received a single injection of 800 μC 3H-glucose. The mice were sacrified at intervals of five minutes until an end time of 240 minutes after giving the labelled glucose. The following results were obtained from morphologic and “semiquantitative” observations: The Golgi vesicles play a prime rôle in the synthesis of mucopolysaccharides. The coupling of the mucopolysaccharides with the proteins take place in the Golgi-cisternae and in the immature secret granules. The existence of two types of Golgi vesicles is discussed.
Zusammenfassung
20 g schwere Mäuse erhielten eine einmalige Dosis von 800 μC 3H-Glukose i.p. injiziert und wurden in Intervallen von 15 bis zu 240 min getötet. Die Silberkornfilamente wurden an den elektronenmikroskopischen Autoradiogrammen ausgezählt und die relativen spezifischen Lokalisationen von 3H-Glukose errechnet.
Den Golgivesikeln kommt bei der Bildung der Mukopolysaccharide eine zentrale Rolle zu. Die Analyse der “seimquantitativen” Resultate ergibt, daß die Koppelung der Mukopolysaccharide mit den Proteinen in den Golgizisternen und in den unreifen Sekretgranula erfolgen muß. Die Befunde lassen die Existenz von zwei Typen von Golgibläschen vermuten: Golgivesikel, denen eine Transportfunktion vom Ergastoplasma zum Golgifeld zukommt, und Golgivesikel, in denen sich die ersten Syntheseschritte der Mukopolysaccharide vollziehen.
Die Verwendbarkeit der 3H-Glukose für autoradiographische Untersuchungen über den Mukopolysaccharidstoffwechsel wird diskutiert.
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Ausgeführt mit Unterstützung durch die Deutsche Forschungsgemeinschaft.
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Schmalbeck, J., Rohr, H. Die Mukopolysaccharid-Synthese in ihrer Beziehung zur Eiweiß-Synthese in der Brunnerschen Drüse der Maus. Zeitschrift für Zellforschung 80, 329–344 (1967). https://doi.org/10.1007/BF00339326
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DOI: https://doi.org/10.1007/BF00339326