Summary
Spheroplasts of Pseudomonas BAL-31/PM2, obtained by treatment of the bacteria with lysozyme, can be infected with purified DNA from bacteriophage PM2. After 4 h of incubation the yield of progeny phage reaches a value of 107-6×107 plaque forming units/μg PM2 DNA. The yield increases linearly with the concentration of DNA over at least 3 orders of magnitude.
The biological activity of double-stranded circular PM2 DNA containing one or more single-strand breaks per molecule (component II), does not differ significantly from that of intact PM2 DNA (component I). Single-stranded PM2 DNA obtained by denaturation of component II, and the irreversible alkali-denatured form of component I are also infective.
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Communicated by P. Starlinger
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van der Schans, G.P., Weyermans, J.P. & Bleichrodt, J.F. Infection of spheroplasts of Pseudomonas with DNA of bacteriophage PM2. Molec. Gen. Genetics 110, 263–271 (1971). https://doi.org/10.1007/BF00337838
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DOI: https://doi.org/10.1007/BF00337838